Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Beom-Ku Han"'
Publikováno v:
Viruses, Vol 16, Iss 6, p 834 (2024)
This study was conducted to efficiently produce virus-like particles (VLPs) of enterovirus 71 (EV71), a causative virus of hand, foot, and mouth disease (HFMD). The expression level of the P1 precursor, a structural protein of EV71, was modified to i
Externí odkaz:
https://doaj.org/article/704497d957a94083bd962883ab3530ca
Autor:
Ki-Back Chu, Hae-Ji Kang, Keon-Woong Yoon, Hae-Ahm Lee, Eun-Kyung Moon, Beom-Ku Han, Fu-Shi Quan
Publikováno v:
Vaccines, Vol 9, Iss 8, p 920 (2021)
The ongoing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic had brought disastrous consequences throughout the entire world. While several manufactured vaccines have been approved for emergency use, continuous efforts to generat
Externí odkaz:
https://doaj.org/article/f201233a532743bf8ddf1eeb607dc2d5
Autor:
Won Seok Gwak, See Nae Lee, Jae Bang Choi, Hyun Soo Kim, Beom Ku Han, Sung Min Bae, Yeon Ho Je, Soo Dong Woo
Publikováno v:
PLoS ONE, Vol 14, Iss 8, p e0221594 (2019)
It is generally accepted that ORF1629 is essential for baculovirus replication, which has enabled isolation of recombinant viruses in a baculovirus expression system using linearized viral DNA. ORF1629-defective viruses cannot replicate in insect cel
Externí odkaz:
https://doaj.org/article/c0a5dc8e526b409c9a56a398df50d621
Effects of repeated burst sequences on the p10 promoter activity of Bombyx mori nucleopolyhedrovirus
Autor:
Beom Ku Han, Soo-Dong Woo, Hyun-Soo Kim, Tae-Hee Kim, Won-Seok Gwak, Jun-Su Bae, Jae Bang Choi
Publikováno v:
Journal of Asia-Pacific Entomology. 24:7-13
The burst sequence (BS) region within the promoter is very important for the burst expression of the very late promoters of baculovirus. As the use of repeated BSs in the polyhedrin promoter was reported to increase its activity, in this study, the e
Publikováno v:
Journal of Biomedical Nanotechnology. 16:1644-1657
Actinobacillus pleuropneumoniaesecretes the hemolytic cytotoxins ApxI, ApxII, ApxIII, and ApxIV, which cause pleurop- neumonia in swine. Of these, ApxI is the most toxic. ApxIA, a repeats-in-toxin toxin-like protein, has strong hemolytic and cytotoxi
Autor:
SeonHyung Lee, Yang-Hoon Kim, Byung-Kwan Cho, Beom-Ku Han, Ji Hun Kim, Sung Min Woo, Ji-Young Ahn, Won-Il Kim
Publikováno v:
Molecular & Cellular Toxicology. 16:263-270
Actinobacillus pleuropneumoniae (A. pleuropneumoniae) produces hemolytic cytotoxins, ApxI, ApxII, ApxIII, and ApxIV, causing a huge loss to the swine industry. We developed the PadLysis protocol for extracting genomic DNA (gDNA) for the LAMP—LeucoC
Publikováno v:
Viruses, Vol 10, Iss 10, p 523 (2018)
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins. Ssp DnaB mini-intein is a useful fusion partner for the production of recombinant proteins becau
Externí odkaz:
https://doaj.org/article/3a4c659882da4c33aead71978d1a8260
Publikováno v:
Microchimica Acta. 188
Nucleic acid amplification tests (NAATs) are powerful tools for the Japanese encephalitis virus (JEV). We demonstrated highly sensitive, specific, and rapid detection of JEV by colorimetric reverse-transcription loop-mediated isothermal amplification
Publikováno v:
Journal of Asia-Pacific Entomology. 21:1079-1084
The baculovirus expression vector system uses the polyhedrin promoter for the expression of foreign proteins. To increase the polyhedrin promoter activity, vectors were constructed by combining two transcription enhancers and the vp39 promoter. The t
Autor:
Won-Suk Gwak, Beom-Ku Han, Tae-Young Shin, Seung Hee Lee, Sung Min Bae, Jae-Bang Choi, Soo-Dong Woo
Publikováno v:
Journal of Asia-Pacific Entomology. 20:591-597
Previously, we found that baculovirus polyhedrin fragments can be successfully used as a fusion partner for the hyper-expression of target proteins. Here, we minimized the fusion fragment of polyhedrin for higher expression of target proteins in a ba