A HUPO test sample study reveals common problems in mass spectrometry–based proteomics

Autor: Michael J. Ellison, Lei Li, John J.M. Bergeron, David W. Speicher, Marina Hincapie, Sjoerd van der Post, Peter J. Sheffield, Catherine C. L. Wong, Thomas A. Beardslee, P. D. Semchuk, Sean C. Bendall, Daniel Cociorva, Wantao Ying, Caroline May, Seul Ki Jeong, Wei Jia, Philip C. Andrews, Steve A. Carr, Benoit Houle, Robert L. Moritz, Lennart Martens, Ana Lopez-Campistrous, Martin Eisenacher, Alexander W. Bell, Raju Kucherlapati, Ron Beavis, Xiaohong Qian, Fuchu He, Brian L. Hood, John R. Yates, Tommy Nilsson, Kieran Wynne, Radoslav Goldman, Vivian Nguyen, Katy Williams, Giuliano Elia, Rudolf Grimm, Salvatore Sechi, Christoph H. Borchers, Yanming An, Masanori Fujimoto, Catherine E. Au, Young Ki Paik, Mahbod Hajivandi, Peipei Ping, Yun Cai, Pavel Metalnikov, Christine A. Miller, Christian Stephan, Wenhong Zhu, Rushdy Ahmad, Marshall Pope, Jinglan Wang, Thomas P. Conrads, Eric W. Deutsch, Min-Seok Kwon, Gilles A. Lajoie, Michael J. Dunn, Guy G. Poirier, Juan Antonio Vizcaíno, William L. Bigbee, Lina Song, John R. Strahler, Sang Yun Cho, Phillip Gray, Craig A. Dorschel, William S. Hancock, Tony Pawson, Kenneth C. Parker, Kaye D. Speicher, Angela K. Walker, Paul F. Predki, Heather Patsiouras, Richard J. Simpson, Thomas Chappell, Kenneth E. Hung, Qing Zhang, Samir M. Hanash, Eugene A. Kapp, Yueju Wang, Katrin Marcus, Jason J. Struthers, Gavin Meredith, Junying Wei, Yangjun Zhang, Sylvie Bourassa, Elisabet Carlsohn, Jayson A. Falkner, Derek Smith, An Sung Chung, Sylvie Laboissiere, Helmut E. Meyer, Hong Wang, Jeffrey W. Smith, Hyoung Joo Lee, David A. Sarracino, Elmar Langenfeld, Kazuyuki Nakamura, Robert E. Kearney, Bing Yang, Bryan Krastins, Majlinda Kullolli

Publikováno v: Nature methods
Biochemistry Publications

We carried out a test sample study to try to identify errors leading to irreproducibility, including incompleteness of peptide sampling, in LC-MS-based proteomics. We distributed a test sample consisting of an equimolar mix of 20 highly purified reco

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4b5e903d9691deb1dd901076a5b4974
https://doi.org/10.1038/nmeth.1333

Tumor-suppressive effect of the retinoic acid receptor beta in human epidermoid lung cancer cells

Autor: Benoit Houle, Walter Edward Bradley, Cécile Rochette-Egly

Publikováno v: Proceedings of the National Academy of Sciences. 90:985-989

Retinoic acid receptor beta (RAR beta), which codes for a nuclear receptor for retinoic acid, is localized in a chromosomal region frequently deleted in lung cancer cells. The gene is expressed in normal lung tissue and in the majority of the cell li

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feffadd49394bbe6719db61ce626985b
https://doi.org/10.1073/pnas.90.3.985

Glucocorticoid ligands specify different interactions with NF-kappaB by allosteric effects on the glucocorticoid receptor DNA binding domain

Autor: Benoit Houle, Adam Stevens, Claire Normand, Andrew Berry, Stuart N. Farrow, David W. Ray, Helen Garside, Barbara Maschera

Publikováno v: The Journal of biological chemistry. 279(48)

Glucocorticoids inhibit inflammation by acting through the glucocorticoid receptor (GR) and powerfully repressing NF-kappaB function. Ligand binding to the C-terminal of GR promotes the nuclear translocation of the receptor and binding to NF-kappaB t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcb63e7d53992f5f65e23ac75db7557c
https://pubmed.ncbi.nlm.nih.gov/15355994

The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS)

Autor: Erik Joly, M G Caron, Luc Ménard, Mireille Legault, Lucie Bertrand, Michel Bouvier, Stephane Angers, Stéphane Parent, Michael S. Brown, Benoit Houle

Publikováno v: Journal of receptor and signal transduction research. 22(1-4)

In BRET2 (Bioluminescence Resonance Energy Transfer), a Renilla luciferase (RLuc) is used as the donor protein, while a Green Fluorescent Protein (GFP2) is used as the acceptor protein. In the presence of the cell permeable substrate DeepBlueC, RLuc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbad989047b55514adda96d8fc4d686c
https://pubmed.ncbi.nlm.nih.gov/12503639

Effects of a tensioned tendon graft in a bone tunnel across the rabbit physis

Autor: Jean-Benoit Houle, Jianping Yang, Merv Letts

Publikováno v: Clinical orthopaedics and related research. (391)

Children who sustain anterior cruciate disruption often are denied the standard reconstructive procedures because of the concern that drilling across the physis of the tibia and femur and compression from a tensioned graft will result in growth plate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c607d2680fc582d4fa9b42a42d44ed29
https://pubmed.ncbi.nlm.nih.gov/11603681