Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Benno Schierling"'
Autor:
Antonio L. Llamas-Saiz, Benno Schierling, José M. Otero, Pablo Guardado-Calvo, Alfred Pingoud, Ann Josée Noël, Wolfgang Wende, Mark J. van Raaij
Publikováno v:
Acta Crystallographica Section F Structural Biology and Crystallization Communications. 68:1139-1148
Enoyl-acyl carrier protein reductase (ENR; the product of the fabI gene) is an important enzyme that is involved in the type II fatty-acid-synthesis pathway of bacteria, plants, apicomplexan protozoa and mitochondria. Harmful pathogens such as Mycoba
Publikováno v:
FEBS Letters. 586:1736-1741
The restriction endonuclease PvuII has been introduced as a sequence-specific cleavage module in highly-specific nucleases for gene targeting. Here, a structural reorganization of the single-chain variant of PvuII (scPvuII) was performed by circular
Autor:
Tatiana S. Oretskaya, Le Thi Hien, Bernhard Spengler, Eugeny Volkov, Alfred Pingoud, Ann-Josée Noël, Michael Kokkinidis, Elena A. Kubareva, Wolfgang Wende, Benno Schierling, Andreas Römpp
Publikováno v:
Proceedings of the National Academy of Sciences. 107:1361-1366
For many applications it would be desirable to be able to control the activity of proteins by using an external signal. In the present study, we have explored the possibility of modulating the activity of a restriction enzyme with light. By cross-lin
Autor:
Volkov Em, Le Thi Hien, A. Pingoud, Benno Schierling, Elena A. Kubareva, Timofei S. Zatsepin, A. Yu. Ryazanova, Tatiana S. Oretskaya, T. I. Velichko
Publikováno v:
Russian Journal of Bioorganic Chemistry. 35:549-555
Derivatives of azobenzene which contained a maleimide group in one of the benzene rings (for binding to a protein cysteine residue) and maleimide, hydroxyl, or carboxyl substitutes in another benzene ring were synthesized. The reactivity of these com
Autor:
Benno, Schierling, Nadine, Dannemann, Lilia, Gabsalilow, Wolfgang, Wende, Toni, Cathomen, Alfred, Pingoud
Publikováno v:
Nucleic Acids Research
Zinc-finger nucleases (ZFNs) typically consist of three to four zinc fingers (ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokI. In this configuration, the ZFs constitute the binding module and the FokI domain the cle
Autor:
Volkov Em, Tatiana S. Oretskaya, Elena A. Kubareva, Timofei S. Zatsepin, Wolfgang Wende, Alfred Pingoud, Benno Schierling, Le Thi Hien
Publikováno v:
Bioconjugate chemistry. 22(7)
A novel method for regulating the activity of homodimeric proteins--"molecular gate" approach--was proposed and its usefulness illustrated for the type II restriction endonuclease SsoII (R.SsoII) as a model. The "molecular gate" approach is based on
Publikováno v:
GBM Fall meeting Hamburg 2007. 2007
Autor:
Benno Schierling, Ann-Josée Noël, Wolfgang Wende, Le Thi Hien, Eugeny Volkava, Elena Kubareva, Oretskaya, Tatiana, Kokkinidis, Michael, Römpp, Andreas, Spengler, Bernhard, Pingoud, Alfred
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America; 1/26/2010, Vol. 107 Issue 4, p1361-1366, 6p