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pro vyhledávání: '"Bennett S. Kalafut"'
Autor:
Bennett S. Kalafut, Koen Visscher
Publikováno v:
Computer Physics Communications. 179:716-723
Biophysical techniques, such as single molecule FRET, fluorescence microscopy, single ion-channel patch clamping, and optical tweezers often yield data that are noisy time series containing discrete steps. Here we present a method enabling objective
Publikováno v:
SPIE Proceedings.
To access the genetic code to be transcribed to RNA, RNA polymerases must first open a “transcription bubble” in the DNA. Structural studies suggest that the minimal model of initiation by T7 bacterophage RNA polymerase (T7 RNAP) consists of two
Autor:
Bennett S. Kalafut, Koen Visscher
Publikováno v:
Biophysical Journal. 102:645a
The RNA homopolymer poly(A) forms single-stranded helices due to base-stacking, the mechanical properties of which may affect the biological function of naturally-occurring A-repeat sequences. Since RNA is a polyanion, screening and binding by cation
Publikováno v:
Biophysical Journal. (4):1034-1041
Gene transcription by the enzyme RNA polymerase is tightly regulated. In many cases, such as in the lac operon in Escherichia coli, this regulation is achieved through the action of protein factors on DNA. Because DNA is an elastic polymer, its respo
Publikováno v:
Biophysical Journal. (3):567a
To access the genetic code to be transcribed to RNA, RNA polymerases must first melt the DNA, opening a “transcription bubble”. In single molecule optical tweezers studies of initiation by T7 bacteriophage RNA polymerase (T7 RNAP), we have found
Autor:
Koen Visscher, Bennett S. Kalafut
Publikováno v:
Biophysical Journal. (3):75a
In addition to serving as the genetic template for protein synthesis in the ribosome, RNA folds in sequence-dependent ways to form structures that have regulatory or catalytic functions. Homopolymeric polyadenylic acid (poly(A))is known to form, due