Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Benjamin I Tickman"'
Autor:
Ian C Nova, Ian M Derrington, Jonathan M Craig, Matthew T Noakes, Benjamin I Tickman, Kenji Doering, Hugh Higinbotham, Andrew H Laszlo, Jens H Gundlach
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0181599 (2017)
Nanopore DNA sequencing is a promising single-molecule analysis technology. This technique relies on a DNA motor enzyme to control movement of DNA precisely through a nanopore. Specific experimental buffer conditions are required based on the preferr
Externí odkaz:
https://doaj.org/article/560b97e04cbb4d4a8f44a96e86638980
Autor:
Jonathan M Craig, Andrew H Laszlo, Ian M Derrington, Brian C Ross, Henry Brinkerhoff, Ian C Nova, Kenji Doering, Benjamin I Tickman, Mark T Svet, Jens H Gundlach
Publikováno v:
PLoS ONE, Vol 10, Iss 11, p e0143253 (2015)
Malyshev et al. showed that the four-letter genetic code within a living organism could be expanded to include the unnatural DNA bases dNaM and d5SICS. However, verification and detection of these unnatural bases in DNA requires new sequencing techni
Externí odkaz:
https://doaj.org/article/03ee6b8236104b3db7ba60539f25f146
Autor:
Widianti Sugianto, Gokce Altin-Yavuzarslan, Benjamin I. Tickman, Cholpisit Kiattisewee, Shuo-Fu Yuan, Sierra M. Brooks, Jitkanya Wong, Hal S. Alper, Alshakim Nelson, James M. Carothers
Publikováno v:
Materials Today Bio, Vol 20, Iss , Pp 100677- (2023)
Engineered living materials (ELMs) fabricated by encapsulating microbes in hydrogels have great potential as bioreactors for sustained bioproduction. While long-term metabolic activity has been demonstrated in these systems, the capacity and dynamics
Externí odkaz:
https://doaj.org/article/c4f2185457494e1da21c785cea4cb26d
Autor:
Jason Fontana, Chen Dong, Cholpisit Kiattisewee, Venkata P. Chavali, Benjamin I. Tickman, James M. Carothers, Jesse G. Zalatan
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
The use of CRISPRa in bacteria lacks predictive rules for identifying effective gRNA target sites. Here the authors define features of bacterial promoters that impose stringent requirements on effective sites.
Externí odkaz:
https://doaj.org/article/8cfc1e2448d44cdbb571ab03ba5acc85
Autor:
Henry Brinkerhoff, Kenji Doering, Matthew T. Noakes, Jonathan W. Mount, Jasmine Bowman, Jens H. Gundlach, Andrew H. Laszlo, Katherine S. Baker, Benjamin I. Tickman, Kyle W. Langford, Ian M. Derrington
Publikováno v:
Nature Biotechnology. 37:651-656
Nanopore DNA sequencing is limited by low base-calling accuracy. Improved base-calling accuracy has so far relied on specialized base-calling algorithms, different nanopores and motor enzymes, or biochemical methods to re-read DNA molecules. Two prim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d0425a658f529d8479a3d7c9f9dc4c1
https://doi.org/10.1038/s41587-019-0096-0
https://doi.org/10.1038/s41587-019-0096-0
Autor:
James M. Carothers, Diego Alba Burbano, Vincent Noireaux, Nicholas S. Kruyer, Benjamin I. Tickman, Pamela Peralta-Yahya, Widianti Sugianto
Publikováno v:
ACS synthetic biology. 10(4)
Membrane proteins are present in a wide array of cellular processes from primary and secondary metabolite synthesis to electron transport and single carbon metabolism. A key barrier to applying membrane proteins industrially is their difficult functi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f2efd93818c77e57963e5fb312be9dc
https://pubmed.ncbi.nlm.nih.gov/33749249
https://pubmed.ncbi.nlm.nih.gov/33749249
Autor:
Vincent Noireaux, Jesse G. Zalatan, Venkata P. Chavali, Aset Khakimzhan, Benjamin I. Tickman, James M. Carothers, Jason Fontana, Diego Alba Burbano, Cholpisit Kiattisewee
Publikováno v:
Cell Systems. 13:215-229.e8
Summary CRISPR-Cas transcriptional circuits hold great promise as platforms for engineering metabolic networks and information processing circuits. Historically, prokaryotic CRISPR control systems have been limited to CRISPRi. Creating approaches to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1834324e5732a86e74cc8cfb0d3d62a8
https://doi.org/10.1016/j.cels.2021.10.008
https://doi.org/10.1016/j.cels.2021.10.008
Autor:
Jason Fontana, James M. Carothers, Aset Khakimzhan, Benjamin I. Tickman, Vincent Noireaux, David Garenne
Publikováno v:
Physical Biology. 18:056003
It is established that for CRISPR-Cas9 applications guide RNAs with 17-20 bp long spacer sequences are optimal for accurate target binding and cleavage. In this work we perform cell-free CRISPRa (CRISPR activation) and CRISPRi (CRISPR inhibition) exp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a558acbc68b336ab39e187bb005a6069
https://doi.org/10.1088/1478-3975/ac091e
https://doi.org/10.1088/1478-3975/ac091e
Autor:
Cholpisit Kiattisewee, James M. Carothers, Jesse G. Zalatan, Chen Dong, Jason Fontana, Benjamin I. Tickman, Venkata P. Chavali
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
In bacterial systems, CRISPR-Cas transcriptional activation (CRISPRa) has the potential to dramatically expand our ability to regulate gene expression, but we lack predictive rules for designing effective gRNA target sites. Here, we identify multiple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8735a45384009aa072b4490f13bc42b
Autor:
Kenji Doering, Henry Brinkerhoff, Noah F. de Leeuw, Matthew T. Noakes, Jonathan M. Craig, Andrew H. Laszlo, Ian M. Derrington, Benjamin I. Tickman, Jens H. Gundlach, Ian C. Nova
Significance DNA helicases are enzymes that use energy from ATP hydrolysis to move along nucleic acid tracks and unwind double-stranded DNA. Helicases are involved in every aspect of DNA metabolism and are therefore vital to maintaining genomic integ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::330ff54702184f279396a8218cf0f6ff
https://europepmc.org/articles/PMC5692567/
https://europepmc.org/articles/PMC5692567/