Nucleosomes impede Cas9 access to DNA in vivo and in vitro

Autor: Max A Horlbeck, Lea B Witkowsky, Benjamin Guglielmi, Joseph M Replogle, Luke A Gilbert, Jacqueline E Villalta, Sharon E Torigoe, Robert Tjian, Jonathan S Weissman

Publikováno v: eLife, Vol 5 (2016)

The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide

Externí odkaz: https://doaj.org/article/0b04a6d7357c482dbd1342412af468f4

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

Autor: Jacqueline E. Villalta, Luke A. Gilbert, Benjamin Guglielmi, Joseph M. Replogle, Robert Tjian, Max A. Horlbeck, Lea B. Witkowsky, Jonathan S. Weissman, Sharon E Torigoe

Publikováno v: eLife, Vol 5 (2016)
eLife

The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08b551e1007b30560a77d76be2cbe628
https://elifesciences.org/articles/12677

TFIIS elongation factor and Mediator act in conjunction during transcription initiation in vivo

Autor: Benjamin Guglielmi, Julie Soutourina, Cyril Esnault, Michel Werner

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2007, 104 (41), pp.16062-7. ⟨10.1073/pnas.0704534104⟩
Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (41), pp.16062-7. ⟨10.1073/pnas.0704534104⟩

The transcription initiation and elongation steps of protein-coding genes usually rely on unrelated protein complexes. However, the TFIIS elongation factor is implicated in both processes. We found that, in the absence of the Med31 Mediator subunit,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2cd7263bcac1743ae36764f31f0189f
https://doi.org/10.1073/pnas.0704534104

A high resolution protein interaction map of the yeast Mediator complex

Autor: Henri-Marc Bourbon, Nynke L. van Berkum, Michel Werner, Muriel Boube, Claire Boschiero, Benjamin Klapholz, Frank C. P. Holstege, Theo Bijma, Benjamin Guglielmi

Publikováno v: Nucleic Acids Research. 32:5379-5391

Mediator is a large, modular protein complex remotely conserved from yeast to man that conveys regulatory signals from DNA-binding transcription factors to RNA polymerase II. In Saccharomyces cerevisiae, Mediator is thought to be composed of 24 subun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::698ab3e8adfc075e29cef05a5227c576
https://doi.org/10.1093/nar/gkh878

Investigating protein-protein interactions in multisubunit proteins: the case of eukaryotic RNA polymerases

Autor: Michel Werner, Benjamin Guglielmi, Pierre Thuriaux, Cécile Zaros

Publikováno v: Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

The physiological functions executed by eukaryotic cells are often catalyzed by large multiprotein complexes. Very few complexes have been analyzed at a detailed structural level, and one therefore strongly relies on generic but indirect methods to e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e15323b59bc50f9474df7e5bbb0624ab
https://doi.org/10.1002/047001153x.g303308