Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Benjamin E. Draper"'
Autor:
Junping Zhang, Dylan A. Frabutt, Matthew Chrzanowski, Ning Li, Lohra M. Miller, Jiahe Tian, Patrick L. Mulcrone, Anh K. Lam, Benjamin E. Draper, Martin F. Jarrold, Roland W. Herzog, Weidong Xiao
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 1, Pp 101206- (2024)
Self-complementary AAV vectors (scAAV) use a mutant inverted terminal repeat (mITR) for efficient packaging of complementary stranded DNA, enabling rapid transgene expression. However, inefficient resolution at the mITR leads to the packaging of mono
Externí odkaz:
https://doaj.org/article/ba0fb73ec32b4ab08090643c484cf170
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 1, Pp 101188- (2024)
Adeno-associated virus (AAV) has shown great promise as a viral vector for gene therapy in clinical applications. The present work studied the effect of genome size on AAV production, purification, and thermostability by producing AAV2-GFP using susp
Externí odkaz:
https://doaj.org/article/79bcb01b76c746f388173992dbe362e4
Autor:
Bryan Troxell, Sarah L. Jaslow, I-Wei Tsai, Chelsea Sullivan, Benjamin E. Draper, Martin F. Jarrold, Kate Lindsey, Levi Blue
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 30, Iss , Pp 288-302 (2023)
Recombinant adeno-associated viruses (rAAVs) deliver DNA to numerous cell types. However, packaging of partial genomes into the rAAV capsid is of concern. Although empty rAAV capsids are studied, there is little information regarding the impact of pa
Externí odkaz:
https://doaj.org/article/8a4925de91024196b5902ddf22890b66
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 27, Iss , Pp 327-336 (2022)
Charge detection mass spectrometry (CDMS) was used to analyze recombinant adeno-associated virus serotype 8 (rAAV8) vectors after incubation at elevated temperatures. rAAV8 vectors with a range of genomes of interest (GOIs) from 2.22 to 4.84 kb were
Externí odkaz:
https://doaj.org/article/3c212eaa5b8041f5a4fd01b527ebcdb2
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 87-97 (2021)
Recombinant adeno-associated virus (rAAV) has emerged as an important gene therapy vector with many clinical trials currently in progress. Analytical characterization and quantitation of particle content remain challenges in both the development and
Externí odkaz:
https://doaj.org/article/28f9114c8b014b91af5eaa66b9638873
Autor:
Elise K. Mullins, Thomas W. Powers, Jim Zobel, Kory M. Clawson, Lauren F. Barnes, Benjamin E. Draper, Qin Zou, Joseph J. Binder, Stanley Dai, Kun Zhang, Olga Friese, Herbert A. Runnels, Martin F. Jarrold, Lawrence C. Thompson
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length. To determine a possible reason for this outcome, we characterized the proportion and compo
Externí odkaz:
https://doaj.org/article/f1f1e75dde024640af7aecb78aac433e
Autor:
Lauren F. Barnes, Benjamin E. Draper, Justin Kurian, Yu-Ting Chen, Tatiana Shapkina, Thomas W. Powers, Martin F. Jarrold
Publikováno v:
Analytical Chemistry. 95:4310-4316
Publikováno v:
Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 87-97 (2021)
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 87-97 (2021)
Recombinant adeno-associated virus (rAAV) has emerged as an important gene therapy vector with many clinical trials currently in progress. Analytical characterization and quantitation of particle content remain challenges in both the development and
Publikováno v:
Analytical chemistry. 94(3)
Adenovirus is one of the largest nonenveloped, double-stranded DNA viruses. It is widely used as a gene therapy vector and has recently received a lot of attention as a novel vaccine platform for SARS-CoV-2. Human adenovirus 5 (HAdV5) contains over 2
Publikováno v:
Analytical chemistry. 93(35)
Vaccines induce immunity by presenting disease antigens through several platforms ranging from individual protein subunits to whole viruses. Due to the large difference in antigen size, the analytical techniques employed for vaccine characterization