Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Benjamin D. Lunstad"'
Autor:
Daniel E. Ryan, Tamar Diamant-Levi, Israel Steinfeld, David Taussig, Savita Visal-Shah, Suhani Thakker, Benjamin D. Lunstad, Robert J. Kaiser, Ryan McCaffrey, Michael Ortiz, Justin Townsend, William R. W. Welch, Mandeep Singh, Bo Curry, Douglas J. Dellinger, Laurakay Bruhn
Publikováno v:
Biochemistry.
CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as optimizing the duration of activity in cells, tissues, and organisms, as well as limiting off-targ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9a0e834fb976f7307540c96931b0e90
https://doi.org/10.1021/acs.biochem.1c00768
https://doi.org/10.1021/acs.biochem.1c00768
Autor:
Benjamin D. Lunstad, Israel Steinfeld, Kenji D Okochi, Laurakay Bruhn, Subhadeep Roy, Daniel E. Ryan, Bo Curry, Magdalena Olesiak, Madhurima Singh, David Taussig, Ryan McCaffrey, Chong Wing Yung, Douglas J. Dellinger, Xuan Vuong, Smruti M Phadnis, Jeffrey R. Sampson
Publikováno v:
Nucleic Acids Research
CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5983ffc5eb2b92f101efcc6c1144cd11
http://europepmc.org/articles/PMC5778453
http://europepmc.org/articles/PMC5778453
Publikováno v:
Org. Biomol. Chem.. 12:1207-1210
RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2cd993319b6ef1a8b54c3917e242290
https://doi.org/10.1039/c3ob42532k
https://doi.org/10.1039/c3ob42532k
Autor:
Subhadeep Roy, Rosa Bacchetta, Rasmus O. Bak, Matthew H. Porteus, Benjamin D. Lunstad, Anya Tsalenko, Ayal Hendel, Israel Steinfeld, Joseph T. Clark, Laurakay Bruhn, Robert J. Kaiser, Douglas J. Dellinger, Alec B. Wilkens, Daniel E. Ryan, Andrew Kennedy
Publikováno v:
Hendel, A, Bak, R O, Clark, J T, Kennedy, A B, Ryan, D E, Roy, S, Steinfeld, I, Lunstad, B D, Kaiser, R J, Wilkens, A B, Bacchetta, R, Tsalenko, A, Dellinger, D, Bruhn, L & Porteus, M H 2015, ' Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells ', Nature Biotechnology . https://doi.org/10.1038/nbt.3290
CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23cc8fdf1bd5b44d01aab22d286377be
https://pure.au.dk/portal/da/publications/chemically-modified-guide-rnas-enhance-crisprcas-genome-editing-in-human-primary-cells(d66dc88c-fd1b-4cde-a6a1-c8a8cc221e53).html
https://pure.au.dk/portal/da/publications/chemically-modified-guide-rnas-enhance-crisprcas-genome-editing-in-human-primary-cells(d66dc88c-fd1b-4cde-a6a1-c8a8cc221e53).html
Autor:
Marvin H. Caruthers, Brian G. Stell, David M Sheehan, Benjamin D. Lunstad, Douglas J. Dellinger, Christina M. Yamada
Publikováno v:
Nucleic Acids Research. 31:4109-4118
Phosphorus-modified phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides were chemically synthesized and their biochemical properties evaluated. Under physiological pH, these DNA analogs possess negative charge and form stable, complem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8d0d2fcd8d20a2f550fe9e34bd09711
https://doi.org/10.1093/nar/gkg439
https://doi.org/10.1093/nar/gkg439
Autor:
Amanda Haas, Pradeep S. Pallan, Eriks Rozners, Devin Leake, Daniel Mutisya, Chelliah Selvam, Martin Egli, Benjamin D. Lunstad
Publikováno v:
Nucleic Acids Research
RNA interference (RNAi) has become an important tool in functional genomics and has an intriguing therapeutic potential. However, the current design of short interfering RNAs (siRNAs) is not optimal for in vivo applications. Non-ionic phosphate backb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5d6638ecfdd2bedf4b80b689100e18e
https://pubmed.ncbi.nlm.nih.gov/24813446
https://pubmed.ncbi.nlm.nih.gov/24813446