Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Benjamin C. Buchmuller"'
Autor:
Benjamin C. Buchmuller, Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Ehud Sass, Emmanuel D. Levy, Michael Knop
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large
Externí odkaz:
https://doaj.org/article/a27b637012fa456695e303da14f899ec
Publikováno v:
ACS Chemical Biology. 18:230-236
Autor:
Himanshu Singh, Chandan K Das, Benjamin C Buchmuller, Lars V Schäfer, Daniel Summerer, Rasmus Linser
Publikováno v:
Nucleic Acids Research.
5-methylcytosine (mC) and its TET-oxidized derivatives exist in CpG dyads of mammalian DNA and regulate cell fate, but how their individual combinations in the two strands of a CpG act as distinct regulatory signals is poorly understood. Readers that
Autor:
Himanshu Singh, Chandan K. Das, Benjamin C. Buchmuller, Simone Eppmann, Lars V. Schäfer, Daniel Summerer, Rasmus Linser
5-methylcytosine (mC) and its TET-oxidized derivatives exist in CpG dyads of mammalian DNA and regulate cell fate, but how their individual combinations in the two strands of a CpG act as distinct regulatory signals is poorly understood. Readers that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::076bb87f57df391471e20e6f430a751a
https://doi.org/10.1101/2022.10.06.511192
https://doi.org/10.1101/2022.10.06.511192
Autor:
Benjamin C. Buchmuller, Jessica Dröden, Himanshu Singh, Shubhendu Palei, Malte Drescher, Rasmus Linser, Daniel Summerer
5-Methylcytosine (mC) and 5-hydroxymethylcytosine (hmC), the two main epigenetic modifications of mammalian DNA, exist in symmetric and asymmetric combinations in the two strands of CpG dyads. However, revealing such combinations in single DNA duplex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b583e9f0432281da722bb85e38e7997a
Autor:
Konrad Herbst, Julia D. Knopf, Julia Fueller, Marius K. Lemberg, Bahtiyar Kurtulmus, Gislene Pereira, Benjamin C. Buchmuller, Daniel Kirrmaier, Michael Knop, Krisztina Gubicza, Matthias Meurer
Publikováno v:
The Journal of Cell Biology
Fueller et al. describe a simple one-step procedure for quick and scalable chromosomal tagging of genes in mammalian cells using PCR products that contain all elements necessary for Cas12a tagging: homology arms and a guide RNA gene for chromosomal i
Autor:
Martin Schäfer, Klaus Kopka, Matthias Eder, Benjamin C. Buchmuller, Karin Leotta, Ulrike Bauder-Wüst, Uwe Haberkorn, Christos Liolios
Publikováno v:
Journal of Medicinal Chemistry. 61:2062-2074
The GRPr, highly expressed in prostate PCa and breast cancer BCa, is a promising target for the development of new PET radiotracers. The chelator HBED-CC (N,N′-bis[2-hydroxy-5-(carboxyethyl)benzyl]ethylenediamine-N,N′-diacetic acid) was coupled t
Autor:
Matthias Meurer, Benjamin C. Buchmuller, Ehud Sass, Konrad Herbst, Emmanuel D. Levy, Michael Knop, Daniel Kirrmaier
Publikováno v:
Nature Communications
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Clone collections of modified strains (“libraries”) are a major resource for systematic studies with the yeast Saccharomyces cerevisiae. Construction of such libraries is time-consuming, costly and confined to the genetic background of a specific
Autor:
Gislene Pereira, Bahtiyar Kurtulmus, Krisztina Gubicza, Matthias Meurer, Konrad Herbst, Benjamin C. Buchmuller, Julia Fueller, Julia D. Knopf, Michael Knop, Daniel Kirrmaier, Marius K. Lemberg
Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides for PCR with generic template cassettes to create linear
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8e01909b2573f07350b22dc1986f8b6
Autor:
Ilia Kats, Martin Štefl, Ehud Sass, Anton Khmelinskii, Verena Dederer, Konrad Herbst, Koen Van Laer, Emmanuel D. Levy, Florian Huber, Tobias P. Dick, Marius K. Lemberg, Benjamin C. Buchmuller, Yuanqiang Duan, Michael Knop, Daniel Kirrmaier, Matthias Meurer
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae ORFs. It consists of 5661 strains with an acceptor module inserted after each ORF, which can be efficiently replaced with tags or regulatory elements. We valida
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::813343810a9ffad87673864af80afe73
https://doi.org/10.1101/226811
https://doi.org/10.1101/226811