Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Benedikt T. Kuhn"'
Autor:
Benedikt T. Kuhn, Jonathan Zöller, Iwan Zimmermann, Tim Gemeinhardt, Dogukan H. Özkul, Julian D. Langer, Markus A. Seeger, Eric R. Geertsma
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)
Abstract Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate via the elevator alternating-access mechanism, and are composed of two rigid domai
Externí odkaz:
https://doaj.org/article/ff069676171e4d63a4d2f96eb75cc12a
Autor:
Eric R. Geertsma, Erich Stefan, Dovile Januliene, Christoph Thomas, Ahmad Reza Mehdipour, Susanne Hofmann, Stefan Brüchert, Arne Moeller, Gerhard Hummer, Benedikt T Kuhn, Robert Tampé
Publikováno v:
Nature
Cryo-electron microscopy (cryo-EM) has the capacity to capture molecular machines in action1-3. ATP-binding cassette (ABC) exporters are highly dynamic membrane proteins that extrude a wide range of substances from the cytosol4-6 and thereby contribu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8ee7ad84202163e23c07e8d4f6d656f
https://doi.org/10.1038/s41586-019-1391-0
https://doi.org/10.1038/s41586-019-1391-0
Autor:
Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A.J. Hutter, Christian Miscenic, Roger J.P. Dawson, Markus A. Seeger, Eric R. Geertsma
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e91f44c161e81d63919ee447e88d400
https://doi.org/10.17504/protocols.io.bpinmkde
https://doi.org/10.17504/protocols.io.bpinmkde
Autor:
Philipp Bräuer, Pascal Egloff, Simon Newstead, Markus A. Seeger, Eric R. Geertsma, Benedikt T Kuhn, Roger J. P. Dawson, Iwan Zimmermann, Cedric A. J. Hutter
Here, we provide a protocol to generate synthetic nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week period. Unlike methods that require animals for antibody generation, sybody selections are carried out en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60fb504f98721c103baafee2d8c5815
https://doi.org/10.5167/uzh-190529
https://doi.org/10.5167/uzh-190529
Autor:
Benedikt T, Kuhn, Iwan, Zimmermann, Pascal, Egloff, Lea M, Hürlimann, Cedric A J, Hutter, Christian, Miscenic, Roger J P, Dawson, Markus A, Seeger, Eric R, Geertsma
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2127
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5668c4a74b9001ba57345ae5cb11be27
https://pubmed.ncbi.nlm.nih.gov/32112321
https://pubmed.ncbi.nlm.nih.gov/32112321
Autor:
Roger J. P. Dawson, Markus A. Seeger, Pascal Egloff, Eric R. Geertsma, Iwan Zimmermann, Cedric A. J. Hutter, Christian Miscenic, Benedikt T Kuhn, Lea M. Hürlimann
Publikováno v:
Methods in Molecular Biology ISBN: 9781071603727
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b424f3c68e9441391fb7b81386de0428
https://www.zora.uzh.ch/id/eprint/196535/
https://www.zora.uzh.ch/id/eprint/196535/
Autor:
Iwan, Zimmermann, Pascal, Egloff, Cedric A J, Hutter, Benedikt T, Kuhn, Philipp, Bräuer, Simon, Newstead, Roger J P, Dawson, Eric R, Geertsma, Markus A, Seeger
Publikováno v:
Nature protocols. 15(5)
Here, we provide a protocol to generate synthetic nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week period. Unlike methods that require animals for antibody generation, sybody selections are carried out en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::439a88dfbcf1c1778881162cd015aa14
https://pubmed.ncbi.nlm.nih.gov/32269381
https://pubmed.ncbi.nlm.nih.gov/32269381
Escherichia coli is one of the most widely used expression hosts for membrane proteins. However, establishing conditions for its recombinant production of membrane proteins remains difficult. Attempts to produce membrane proteins frequently result in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53b2ffa145b6bdd8e252709ec275a0e2
https://www.zora.uzh.ch/id/eprint/139760/
https://www.zora.uzh.ch/id/eprint/139760/