Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair

Autor: Wei-Ting Lu, Ben R. Hawley, George L. Skalka, Robert A. Baldock, Ewan M. Smith, Aldo S. Bader, Michal Malewicz, Felicity Z. Watts, Ania Wilczynska, Martin Bushell

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)

The mechanism through which Drosha and Dicer affect DNA repair is not clear. Here the authors use a high-throughput approach to uncover the role of Drosha in promoting DNA:RNA hybrids at DNA damaged sites.

Externí odkaz: https://doaj.org/article/4f06c76c0d594080bebcce6e6a2b7046

DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci

Autor: Janna Luessing, Aldo S. Bader, Wei-Ting Lu, George Skalka, Ben R Hawley, Noel F. Lowndes, Martin Bushell

Proteins with RNA-binding activity are increasingly being implicated in DNA damage responses (DDR). Additionally, DNA:RNA-hybrids are rapidly generated around DNA double-strand breaks (DSBs), and are essential for effective repair. Here, using a meta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b78dc5f1a2054e8607f798b77c459c1
https://www.repository.cam.ac.uk/handle/1810/344182

The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112

Autor: Christiane Zorbas, Samie R. Jaffrey, Nathalie Ulryck, Katherine E. Bohnsack, Marc Graille, Philipp Hackert, Felix G.M. Ernst, Denis L. J. Lafontaine, Ben R Hawley, Markus T. Bohnsack, Nhan van Tran

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2019, 47 (15), pp.7719-7733. ⟨10.1093/nar/gkz619⟩
Nucleic acids research, 47 (15

N6-methyladenosine (m6A) has recently been found abundantly on messenger RNA and shown to regulate most steps of mRNA metabolism. Several important m6A methyltransferases have been described functionally and structurally, but the enzymes responsible

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfed45ba4c5d626e2a904ee303136af0
https://hal-polytechnique.archives-ouvertes.fr/hal-02333696/file/Tran-NAR-2019-HsMETTL5-TRMT112.pdf

The roles of RNA in DNA double-strand break repair

Autor: Ania Wilczynska, Ben R Hawley, Martin Bushell, Aldo S. Bader

Publikováno v: British Journal of Cancer

Effective DNA repair is essential for cell survival: a failure to correctly repair damage leads to the accumulation of mutations and is the driving force for carcinogenesis. Multiple pathways have evolved to protect against both intrinsic and extrins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::173a16cec5f0d74f69054f1aa9e0191d
https://pubmed.ncbi.nlm.nih.gov/31894141

FTO controls reversible m(6)Am RNA methylation during snRNA biogenesis

Autor: Livio Pellizzoni, Steven S. Gross, Jan Mauer, Hani Goodarzi, Samie R. Jaffrey, Ben R Hawley, Miriam Sindelar, Théo Guez, Françoise Debart, Vladimir Despic, Andrea Rentmeister, Jean-Jacques Vasseur

Publikováno v: Nature Chemical Biology
Nature Chemical Biology, Nature Publishing Group, 2019, 15 (4), pp.340-347. ⟨10.1038/s41589-019-0231-8⟩
Nature chemical biology, vol 15, iss 4

Small nuclear RNAs (snRNAs) are core spliceosome components and mediate pre-mRNA splicing. Here we show that snRNAs contain a regulated and reversible nucleotide modification causing them to exist as two different methyl isoforms, m1 and m2, reflecti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::500c15071dbb0665f58f76323669ceac
https://europepmc.org/articles/PMC6984009/