Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens

Autor: Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Guillaume Le Heiget, Sandra Pizzut, Hanna Kulyk, Floriant Bellvert, Sophie Bozonnet, Laurence A. Mulard, Magali Remaud-Siméon, Claire Moulis, Isabelle André

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme a

Externí odkaz: https://doaj.org/article/ef2c88e7c7fc466baca624798a644eb8

Combining multi-scale modelling methods to decipher molecular motions of a branching sucrase from glycoside-hydrolase family 70.

Autor: Akli Ben Imeddourene, Jérémy Esque, Isabelle André

Publikováno v: PLoS ONE, Vol 13, Iss 8, p e0201323 (2018)

Among α-transglucosylases from Glycoside-Hydrolase family 70, the ΔN123-GB-CD2 enzyme derived from the bifunctional DSR-E from L. citreum NRRL B-1299 is particularly interesting as it was the first described engineered Branching Sucrase, not able t

Externí odkaz: https://doaj.org/article/9b0a56a25c1c4a1ba1c99dea99f29f49

Simulations Meet Experiment to Reveal New Insights into DNA Intrinsic Mechanics.

Autor: Akli Ben Imeddourene, Ahmad Elbahnsi, Marc Guéroult, Christophe Oguey, Nicolas Foloppe, Brigitte Hartmann

Publikováno v: PLoS Computational Biology, Vol 11, Iss 12, p e1004631 (2015)

The accurate prediction of the structure and dynamics of DNA remains a major challenge in computational biology due to the dearth of precise experimental information on DNA free in solution and limitations in the DNA force-fields underpinning the sim

Externí odkaz: https://doaj.org/article/6976a8e8185c48eab154ac6ca98fdbf7

A convergent chemoenzymatic strategy to deliver a diversity of Shigella flexneri serotype-specific O-antigen segments from a unique lightly protected tetrasaccharide core

Autor: Akli Ben Imeddourene, Guillaume Le Heiget, Claire Moulis, Isabelle André, Zhaoyu Hu, Nelly Monties, Laurence A. Mulard, Magali Remaud-Simeon, Mounir Benkoulouche, Louis-Antoine Barel, Catherine Guerreiro, Yann Le Guen

Publikováno v: Journal of Organic Chemistry
Journal of Organic Chemistry, 2021, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩
Journal of Organic Chemistry, American Chemical Society, 2020, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩

International audience; Progress in glycoscience is strongly dependent on the availability of broadly diverse tailored-made, well-defined and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical bou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9424a376996bb908628ac2d5c4f976e
https://hal.insa-toulouse.fr/hal-03020947/document

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