Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Autor: Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Dorian Lefebvre, Mathieu Fanuel, Hélène Rogniaux, David Ropartz, Sophie Barbe, David Guieysse, Laurence A. Mulard, Magali Remaud-Siméon, Claire Moulis, Isabelle André

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract Enzyme engineering approaches have allowed to extend the collection of enzymatic tools available for synthetic purposes. However, controlling the regioselectivity of the reaction remains challenging, in particular when dealing with carbohydr

Externí odkaz: https://doaj.org/article/6fca7743f5704259be614afb146d57e5

Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides.

Autor: Benkoulouche, Mounir¹ (AUTHOR), Ben Imeddourene, Akli¹ (AUTHOR), Barel, Louis-Antoine^2,3 (AUTHOR), Lefebvre, Dorian¹ (AUTHOR), Fanuel, Mathieu^4,5 (AUTHOR), Rogniaux, Hélène^4,5 (AUTHOR), Ropartz, David^4,5 (AUTHOR), Barbe, Sophie¹ (AUTHOR), Guieysse, David¹ (AUTHOR), Mulard, Laurence A.² (AUTHOR), Remaud-Siméon, Magali¹ (AUTHOR), Moulis, Claire¹ (AUTHOR), André, Isabelle¹ (AUTHOR) isabelle.andre@insa-toulouse.fr

Publikováno v: Scientific Reports. 10/13/2021, Vol. 11 Issue 1, p1-14. 14p.

Combining multi-scale modelling methods to decipher molecular motions of a branching sucrase from glycoside-hydrolase family 70.

Autor: Akli Ben Imeddourene, Jérémy Esque, Isabelle André

Publikováno v: PLoS ONE, Vol 13, Iss 8, p e0201323 (2018)

Among α-transglucosylases from Glycoside-Hydrolase family 70, the ΔN123-GB-CD2 enzyme derived from the bifunctional DSR-E from L. citreum NRRL B-1299 is particularly interesting as it was the first described engineered Branching Sucrase, not able t

Externí odkaz: https://doaj.org/article/9b0a56a25c1c4a1ba1c99dea99f29f49

Simulations Meet Experiment to Reveal New Insights into DNA Intrinsic Mechanics.

Autor: Akli Ben Imeddourene, Ahmad Elbahnsi, Marc Guéroult, Christophe Oguey, Nicolas Foloppe, Brigitte Hartmann

Publikováno v: PLoS Computational Biology, Vol 11, Iss 12, p e1004631 (2015)

The accurate prediction of the structure and dynamics of DNA remains a major challenge in computational biology due to the dearth of precise experimental information on DNA free in solution and limitations in the DNA force-fields underpinning the sim

Externí odkaz: https://doaj.org/article/6976a8e8185c48eab154ac6ca98fdbf7

A convergent chemoenzymatic strategy to deliver a diversity of Shigella flexneri serotype-specific O-antigen segments from a unique lightly protected tetrasaccharide core

Autor: Akli Ben Imeddourene, Guillaume Le Heiget, Claire Moulis, Isabelle André, Zhaoyu Hu, Nelly Monties, Laurence A. Mulard, Magali Remaud-Simeon, Mounir Benkoulouche, Louis-Antoine Barel, Catherine Guerreiro, Yann Le Guen

Publikováno v: Journal of Organic Chemistry
Journal of Organic Chemistry, 2021, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩
Journal of Organic Chemistry, American Chemical Society, 2020, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩

International audience; Progress in glycoscience is strongly dependent on the availability of broadly diverse tailored-made, well-defined and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical bou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9424a376996bb908628ac2d5c4f976e
https://hal.insa-toulouse.fr/hal-03020947/document