Zobrazeno 1 - 10
of 82
pro vyhledávání: '"Belur N Manjula"'
Autor:
James M. Manning, A. Seetharama Acharya, Vincent A. Fischetti, Alberto Di Donato, Belur N. Manjula
Publikováno v:
International Journal of Peptide and Protein Research. 22:78-82
The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecc4c2239323d5a36ed4a9d5939adc00
https://doi.org/10.1111/j.1399-3011.1983.tb02071.x
https://doi.org/10.1111/j.1399-3011.1983.tb02071.x
Publikováno v:
Biochemistry. 47:10981-10990
The PEGylated hemoglobin (Hb) has been evaluated as a potential blood substitute. In an attempt to understand the autoxidation of the PEGylated Hb, we have studied the autoxidation of the PEGylated Hb site-specifically modified at Cys-93(beta) or at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a924112b9171944eb1d6603d000037e7
https://doi.org/10.1021/bi800906z
https://doi.org/10.1021/bi800906z
Publikováno v:
Analytical Biochemistry. 374:231-242
The design of the extension arm-facilitated PEGylation (EAFP) of proteins takes advantage of the high selective and quantitative aspects of the thiol-maleimide reaction. However, the efficiency of EAFP with hemoglobin varied with the batches of malei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04d84fc7f9e0a7e23cf84284710854d2
https://doi.org/10.1016/j.ab.2007.11.034
https://doi.org/10.1016/j.ab.2007.11.034
Autor:
Seetharama A. Acharya, Marcos Intaglietta, Belur N. Manjula, Amy G. Tsai, Vivek N. Acharya, Nirmala D. Kanika
Publikováno v:
Biochemical Journal. 405:503-511
TetraPEGylated canine Hb, [SP (succinimidophenyl)-PEG5K]4-canine-Hb, with PEGylation at its four reactive cysteine residues (α111 and β93) has been prepared and characterized. The hydrodynamic volume and the molecular radius of (SP-PEG5K)4-canine-H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdeab9d5a71c2c02c0dc61f1eb09cb39
https://doi.org/10.1042/bj20070238
https://doi.org/10.1042/bj20070238
Publikováno v:
Artificial Cells, Blood Substitutes, and Biotechnology. 35:107-118
The PEGylation that adds an extension arm on protein amino groups with the conservation of their positive charge masks the A and D antigens of erythrocytes efficiently. In the present study, the efficiency of masking the antigens of RBC by PEGylation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24c9dc5a97b9e087099cb3a9e6e20c09
https://doi.org/10.1080/10731190600974657
https://doi.org/10.1080/10731190600974657
Autor:
Belur N. Manjula, Pedro Cabrales, Seetharama A. Acharya, Marcos Intaglietta, Robert M. Winslow, Amy G. Tsai
Publikováno v:
Blood. 108:3603-3610
Cell-free hemoglobin's (CFH) high affinity for nitric oxide (NO) could limit CFH's use as an oxygen-carrying blood replacement fluid because it scavenges NO, causing vasoconstriction and hypertension. However, the extent to which perivascular NO leve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72f31b2d487ff8cd976ecb1f08e0967c
https://doi.org/10.1182/blood-2006-02-005272
https://doi.org/10.1182/blood-2006-02-005272
Autor:
Seetharama A. Acharya, Marcos Intaglietta, Belur N. Manjula, Amy G. Tsai, Pedro Cabrales, Parimala Nacharaju, Nanae Hangai-Hoger
Publikováno v:
Critical Care Medicine. 34:108-117
Objective: To determine whether resuscitation with polyethylene glycol conjugated bovine serum albumin (2.5% weight/volume) infused at 16 mL/kg/hr (PEG-BSA-16) or at 24 mL/kg/hr (PEG-BSA-24) for 1 hr improves microcirculatory conditions in endotoxemi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e7d1cde8e112b48c18c9ffd0c17d168
https://doi.org/10.1097/01.ccm.0000190623.97200.82
https://doi.org/10.1097/01.ccm.0000190623.97200.82
Publikováno v:
Artificial Cells, Blood Substitutes, and Biotechnology. 34:381-393
Surface decoration of hemoglobin (Hb) with six copies of PEG-5K employing thiolation mediated PEGylation platform neutralizes the vasoaconstricive activity of acellular Hb. The molecular size homogeneity of hexaPEGylated Hb, in spite of the fact that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4a10e8cfd331bf5c820499b6aaa0437
https://doi.org/10.1080/10731190600769164
https://doi.org/10.1080/10731190600769164
Publikováno v:
Transfusion. 45:374-383
BACKGROUND: The surface decoration of red blood cells (RBCs) by polyethylene glycol (PEG) chains has been an approach developed to camouflage the blood group antigens from their antibodies. A PEGylation protocol, however, that can mask the antigens a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f992b9c8a7c8c0fffa086b8479d80d79
https://doi.org/10.1111/j.1537-2995.2005.04290.x
https://doi.org/10.1111/j.1537-2995.2005.04290.x
Autor:
Seetharama A. Acharya, Joel M. Friedman, Eric S. Peterson, Belur N. Manjula, Imran Khan, Celia Bonaventura, Laura Juczszak, Jiaqian Wang, Roman Shinder
Publikováno v:
Biochemistry. 43:4832-4843
The water-filled central cavity of human adult hemoglobin (Hb A) is the binding or interaction site for many different allosteric effectors. Oxygen binding titrations reveal that pyrenetetrasulfonate (PyTS), a fluorescent analogue of 2,3-diphosphogly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8395889ed0532c82ede51cd83b749efa
https://doi.org/10.1021/bi035481o
https://doi.org/10.1021/bi035481o