Distinctive profiles of small RNA couple inverted repeat-induced post-transcriptional gene silencing with endogenous RNA silencing pathways in Arabidopsis

Autor: Richard W Michelmore, Manjula Govindarajulu, Joan Wong, Marta Matvienko, Huaqin Xu, Urszula Piskurewicz, Tadeusz Wroblewski, Alexander Kozik, Belinda Martineau

Publikováno v: RNA (New York, N.Y.), vol 20, iss 12

The experimental induction of RNA silencing in plants often involves expression of transgenes encoding inverted repeat (IR) sequences to produce abundant dsRNAs that are processed into small RNAs (sRNAs). These sRNAs are key mediators of post-transcr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5c31f919a66d429778c179322d48027
https://europepmc.org/articles/PMC4238362/

Consequences of normalizing transcriptomic and genomic libraries of plant genomes using a duplex-specific nuclease and tetramethylammonium chloride

Autor: Bertrand Perroud, Richard W Michelmore, Lutz Froenicke, Marta Matvienko, Belinda Martineau, Alexander Kozik, Dean Lavelle

Publikováno v: PLoS ONE, Vol 8, Iss 2, p e55913 (2013)
PLoS ONE

Several applications of high throughput genome and transcriptome sequencing would benefit from a reduction of the high-copy-number sequences in the libraries being sequenced and analyzed, particularly when applied to species with large genomes. We ad

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cafd44cc49ee761e97c69e9fd33e2f1
http://europepmc.org/articles/PMC3568094?pdf=render

Regulatory assessment of the FLAVR SAVR tomato

Autor: Belinda Martineau, Donald Emlay, William R. Hiatt, Keith Redenbaugh

Publikováno v: Trends in Food Science & Technology. 5:105-110

This article provides a case study of the FLAVR SAVR☆m tomato as a demonstration of the regulatory procedures and safety assessment necessary in the USA. The data generated demonstrate that FLAVR SAVR tomatoes do not significantly differ from tradi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e9efa8612d7d63953fbbd0ca5ee01cb9
https://doi.org/10.1016/0924-2244(94)90197-x

An efficient and reliable method for determining the number of transgenes inserted into transgenic plants

Autor: Belinda Martineau, Rick A. Sanders, Raymond E. Sheehy

Publikováno v: Plant Molecular Biology Reporter. 10:164-172

A simple and reliable method is described for determining the number of transgenes inserted into transgenic plants. Analysis of tomato plants transformed with an antisense polygalacturonase gene is used to demonstrate the method. The use of the metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::451ee5a703be507ff635806b47665f6b
https://doi.org/10.1007/bf02668343

In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries

Autor: David G. Gilchrist, Richard M. Bostock, James E. Lincoln, Saskia A. Hogenhout, Alan B. Bennett, Belinda Martineau, Erik E. Karrer, William J. Lucas, Danny C. Alexander

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America 92 (1995) 9
Proceedings of the National Academy of Sciences of the United States of America, 92(9), 3814-3818

A method for isolating and cloning mRNA populations from individual cells in living, intact plant tissues is described. The contents of individual cells were aspirated into micropipette tips filled with RNA extraction buffer. The mRNA from these cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03803bf05ac7e8b869011b510242f7ba
https://europepmc.org/articles/PMC42052/