Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Behrouz Eslami Mossallam"'
Autor:
Behrouz Eslami-Mossallam, Misha Klein, Constantijn V. D. Smagt, Koen V. D. Sanden, Stephen K. Jones, John A. Hawkins, Ilya J. Finkelstein, Martin Depken
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Cas9 off-target sites can be predicted by many bioinformatics tools. Here the authors present low complexity mechanistic model that characterizes SpCas9 kinetics in free-energy terms, allowing quantitative prediction of off-target activity in bulk-bi
Externí odkaz:
https://doaj.org/article/8ce17f4dfe3843928f961470b5546a13
Publikováno v:
Cell Reports, Vol 22, Iss 6, Pp 1413-1423 (2018)
Summary: Due to their specificity, efficiency, and ease of programming, CRISPR-associated nucleases are popular tools for genome editing. On the genomic scale, these nucleases still show considerable off-target activity though, posing a serious obsta
Externí odkaz:
https://doaj.org/article/8930b2f12fd84f7187b4303a1fcd6861
Publikováno v:
PLoS ONE, Vol 11, Iss 6, p e0156905 (2016)
Eukaryotic DNA is strongly bent inside fundamental packaging units: the nucleosomes. It is known that their positions are strongly influenced by the mechanical properties of the underlying DNA sequence. Here we discuss the possibility that these mech
Externí odkaz:
https://doaj.org/article/9d91d39fe1994feaba1cebd3e23f7e1c
Publikováno v:
Building and Environment. 228:109839
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eccba99cc16eec8fa3e3552f50f870a2
https://doi.org/10.1016/j.buildenv.2022.109839
https://doi.org/10.1016/j.buildenv.2022.109839
Autor:
Richard Janissen, Behrouz Eslami-Mossallam, Irina Artsimovitch, Martin Depken, Nynke H. Dekker
Publikováno v:
Cell Reports, 39(4)
Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our mechanistic understan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50115ecc6840d564199ed3f365cea03a
https://pubmed.ncbi.nlm.nih.gov/35476989
https://pubmed.ncbi.nlm.nih.gov/35476989
Autor:
Richard Janissen, Behrouz Eslami-Mossallam, Irina Artsimovitch, Martin Depken, Nynke H. Dekker
Publikováno v:
SSRN Electronic Journal.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8fab848b0bebaf8bf9075ae90b6f4c4c
https://doi.org/10.2139/ssrn.3937610
https://doi.org/10.2139/ssrn.3937610
Autor:
Behrouz, Eslami-Mossallam, Misha, Klein, Constantijn V D, Smagt, Koen V D, Sanden, Stephen K, Jones, John A, Hawkins, Ilya J, Finkelstein, Martin, Depken
Publikováno v:
Nature communications. 13(1)
The S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool. SpCas9 is directed to target sites based on complementarity to a complexed single-guide RNA (sgRNA). However, SpCas9-sgRNA also binds and cleaves genomic off-targets with only
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7269eec35d9cd050cb723c30b22dead2
https://pubmed.ncbi.nlm.nih.gov/35292641
https://pubmed.ncbi.nlm.nih.gov/35292641
Autor:
Stephen K. Jones, Misha Klein, Behrouz Eslami-Mossallam, Martin Depken, John A. Hawkins, Constantijn van der Smagt, Koen van der Sanden, Ilya J. Finkelstein
The SpCas9 endonuclease has become an important tool in gene-editing and basic science alike. Though easily programmed to target any sequence, SpCas9 also shows considerable activity over genomic off-targets. Many empirical facts regarding the target
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbb3456a08a4aefb2bf9d52ec9c1a1ed
https://doi.org/10.1101/2020.05.21.108613
https://doi.org/10.1101/2020.05.21.108613
Autor:
Irina Artsimovitch, Nynke H. Dekker, Richard Janissen, Behrouz Eslami-Mossallam, Martin Depken
Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our understanding of back
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::87df372cbf25692daf7e73abd475edbd
https://doi.org/10.1101/762468
https://doi.org/10.1101/762468
Publikováno v:
The Journal of Physical Chemistry B. 120:5855-5863
Nucleosomes, DNA spools with a protein core, engage about three-quarters of eukaryotic DNA and play a critical role in chromosomal processes, ranging from gene regulation, recombination, and replication to chromosome condensation. For more than a dec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bf6872117ff31207b918a556665d8ac
https://doi.org/10.1021/acs.jpcb.6b00391
https://doi.org/10.1021/acs.jpcb.6b00391