Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Behrouz Eslami"'
Autor:
Behrouz Eslami-Mossallam, Misha Klein, Constantijn V. D. Smagt, Koen V. D. Sanden, Stephen K. Jones, John A. Hawkins, Ilya J. Finkelstein, Martin Depken
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Cas9 off-target sites can be predicted by many bioinformatics tools. Here the authors present low complexity mechanistic model that characterizes SpCas9 kinetics in free-energy terms, allowing quantitative prediction of off-target activity in bulk-bi
Externí odkaz:
https://doaj.org/article/8ce17f4dfe3843928f961470b5546a13
Publikováno v:
طب اورژانس ایران, Pp e32-e32 (2020)
Introduction: The main goal of health system reform plan, was to provide financial support for health costs of citizens, organize hospital services and improve their quality, and provide fair access to health care. Therefore, this study aimed to eval
Externí odkaz:
https://doaj.org/article/c208c534e65a4bc88a6b2bdaf38664cb
Publikováno v:
Cell Reports, Vol 22, Iss 6, Pp 1413-1423 (2018)
Summary: Due to their specificity, efficiency, and ease of programming, CRISPR-associated nucleases are popular tools for genome editing. On the genomic scale, these nucleases still show considerable off-target activity though, posing a serious obsta
Externí odkaz:
https://doaj.org/article/8930b2f12fd84f7187b4303a1fcd6861
Publikováno v:
PLoS ONE, Vol 11, Iss 6, p e0156905 (2016)
Eukaryotic DNA is strongly bent inside fundamental packaging units: the nucleosomes. It is known that their positions are strongly influenced by the mechanical properties of the underlying DNA sequence. Here we discuss the possibility that these mech
Externí odkaz:
https://doaj.org/article/9d91d39fe1994feaba1cebd3e23f7e1c
Publikováno v:
Building and Environment. 228:109839
Autor:
Richard Janissen, Behrouz Eslami-Mossallam, Irina Artsimovitch, Martin Depken, Nynke H. Dekker
Publikováno v:
Cell Reports, 39(4)
Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our mechanistic understan
Autor:
Richard Janissen, Behrouz Eslami-Mossallam, Irina Artsimovitch, Martin Depken, Nynke H. Dekker
Publikováno v:
SSRN Electronic Journal.
Autor:
Behrouz, Eslami-Mossallam, Misha, Klein, Constantijn V D, Smagt, Koen V D, Sanden, Stephen K, Jones, John A, Hawkins, Ilya J, Finkelstein, Martin, Depken
Publikováno v:
Nature communications. 13(1)
The S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool. SpCas9 is directed to target sites based on complementarity to a complexed single-guide RNA (sgRNA). However, SpCas9-sgRNA also binds and cleaves genomic off-targets with only
Autor:
Stephen K. Jones, Misha Klein, Behrouz Eslami-Mossallam, Martin Depken, John A. Hawkins, Constantijn van der Smagt, Koen van der Sanden, Ilya J. Finkelstein
The SpCas9 endonuclease has become an important tool in gene-editing and basic science alike. Though easily programmed to target any sequence, SpCas9 also shows considerable activity over genomic off-targets. Many empirical facts regarding the target
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbb3456a08a4aefb2bf9d52ec9c1a1ed
https://doi.org/10.1101/2020.05.21.108613
https://doi.org/10.1101/2020.05.21.108613
Autor:
Irina Artsimovitch, Nynke H. Dekker, Richard Janissen, Behrouz Eslami-Mossallam, Martin Depken
Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our understanding of back
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::87df372cbf25692daf7e73abd475edbd
https://doi.org/10.1101/762468
https://doi.org/10.1101/762468