Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Becky Provinciael"'
Publikováno v:
BioTechniques, Vol 72, Iss 6, Pp 245-254 (2022)
Basic and antiviral research on SARS-CoV-2 rely on cellular assays of virus replication in vitro. In addition, accurate detection of virus-infected cells and released virus particles is needed to study virus replication and to profile new candidate a
Externí odkaz:
https://doaj.org/article/d818019b274b4772a5986da5d93e90ca
Autor:
Emiel Vanhulle, Thomas D’huys, Becky Provinciael, Joren Stroobants, Anita Camps, Sam Noppen, Dominique Schols, Els J. M. Van Damme, Piet Maes, Annelies Stevaert, Kurt Vermeire
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 12 (2022)
Urtica dioica agglutinin (UDA) is a carbohydrate-binding small monomeric protein isolated from stinging nettle rhizomes. It inhibits replication of a broad range of viruses, including coronaviruses, in multiple cell types, with appealing selectivity.
Externí odkaz:
https://doaj.org/article/b8bea83cab5e4494bd4d230d23e683a9
Autor:
Kelly Bugatti, Andrea Sartori, Lucia Battistini, Crescenzo Coppa, Emiel Vanhulle, Sam Noppen, Becky Provinciael, Lieve Naesens, Annelies Stevaert, Alessandro Contini, Kurt Vermeire, Franca Zanardi
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 10, p 8765 (2023)
Though the bulk of the COVID-19 pandemic is behind, the search for effective and safe anti-SARS-CoV-2 drugs continues to be relevant. A highly pursued approach for antiviral drug development involves targeting the viral spike (S) protein of SARS-CoV-
Externí odkaz:
https://doaj.org/article/f60413dfa7c94c51ab033a9e6be82aaf
Autor:
Elisa Claeys, Eva Pauwels, Stephanie Humblet-Baron, Becky Provinciael, Dominique Schols, Mark Waer, Ben Sprangers, Kurt Vermeire
Publikováno v:
Frontiers in Immunology, Vol 12 (2021)
The small molecule cyclotriazadisulfonamide (CADA) down-modulates the human CD4 receptor, an important factor in T cell activation. Here, we addressed the immunosuppressive potential of CADA using different activation models. CADA inhibited lymphocyt
Externí odkaz:
https://doaj.org/article/4e52bcb39c314f059ca28d159532c3dd
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 2, p 584 (2022)
One of the reported substrates for the endoplasmic reticulum (ER) translocation inhibitor cyclotriazadisulfonamide (CADA) is DNAJC3, a chaperone of the unfolded protein response during ER stress. In this study, we investigated the impact of altered D
Externí odkaz:
https://doaj.org/article/8167d431a1cb4f338c77596224b9c919
Autor:
Eva Pauwels, Neesha R. Shewakramani, Brent De Wijngaert, Anita Camps, Becky Provinciael, Joren Stroobants, Kai-Uwe Kalies, Enno Hartmann, Piet Maes, Kurt Vermeire, Kalyan Das
Publikováno v:
Science Advances. 9
During co-translational translocation, the signal peptide of a nascent chain binds Sec61 translocon to initiate protein transport through the ER membrane. Our cryo-EM structure of ribosome-Sec61 shows binding of an ordered heterotetrameric TRransloco
Autor:
Wolfgang Klein, Claudia Rutz, Jamina Eckhard, Becky Provinciael, Edgar Specker, Martin Neuenschwander, Gunnar Kleinau, Patrick Scheerer, Jens-Peter von Kries, Marc Nazaré, Kurt Vermeire, Ralf Schülein
Publikováno v:
PLoS ONE, Vol 13, Iss 12, p e0208641 (2018)
The SRP-Sec61 targeting/translocation pathway of eukaryotic cells targets nascent protein chains to the membrane of the endoplasmic reticulum. Using this machinery, secretory proteins are translocated across this membrane whereas membrane proteins ar
Externí odkaz:
https://doaj.org/article/e976463e5a704c7389c9d62dead9c3a8
Autor:
Emiel Vanhulle, Jordi Doijen, Joren Stroobants, Becky Provinciael, Sam Noppen, Dominique Schols, Annelies Stevaert, Kurt Vermeire
Publikováno v:
Antiviral Research. 213:105587
Despite the vaccination campaigns for COVID-19, we still cannot control the spread of SARS-CoV-2, as evidenced by the ongoing circulation of the Omicron variants of concern. This highlights the need for broad-spectrum antivirals to further combat COV
Autor:
Emiel Vanhulle, Joren Stroobants, Becky Provinciael, Anita Camps, Sam Noppen, Piet Maes, Kurt Vermeire
Despite the great success of the administered vaccines against SARS-CoV-2, the virus can still spread, as evidenced by the current circulation of the highly contagious Omicron variant. This emphasizes the additional need to develop effective antivira
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c8a8c2abff1446049b1717ec2e68489
https://lirias.kuleuven.be/handle/20.500.12942/696170
https://lirias.kuleuven.be/handle/20.500.12942/696170
Autor:
Eva Pauwels, Kai-Uwe Kalies, Dominique Schols, Kurt Vermeire, Enno Hartmann, Victor Van Puyenbroeck, Thomas W. Bell, Becky Provinciael
Publikováno v:
Traffic. 21:250-264
Cyclotriazadisulfonamide (CADA) inhibits the co-translational translocation of human CD4 (huCD4) into the endoplasmic reticulum lumen in a signal peptide (SP)-dependent way. We propose that CADA binds the nascent huCD4 SP in a folded conformation wit