Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Beckley K. Nfor"'
Autor:
Martijn W. H. Pinkse, Beckley K. Nfor, Marcel Ottens, Peter Verhaert, Alexander T. Hanke, Frieder Kröner, Jürgen Hubbuch
Publikováno v:
Journal of Chromatography A. 1311:55-64
The accelerating growth of the market for proteins and the growing interest in new, more complex molecules are bringing new challenges to the downstream process development of these proteins. This results in a demand for faster, more cost efficient,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a2068e4f271f683fec0a4be3d0e75a3
https://doi.org/10.1016/j.chroma.2013.08.034
https://doi.org/10.1016/j.chroma.2013.08.034
Publikováno v:
Biotechnology Journal, 7(10), 1221-1232
Biotechnology Journal 7 (2012) 10
Biotechnology Journal 7 (2012) 10
In this study, an integrated approach involving the combined use of high-throughput screening (HTS) and column modeling during process development was applied to an industrial case involving the evaluation of four anion-exchange chromatography (AEX)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81abff605f66ef8836f9791c78bbbf53
https://doi.org/10.1002/biot.201200191
https://doi.org/10.1002/biot.201200191
Autor:
Marcel Ottens, Beckley K. Nfor, Peter Verhaert, Luuk A.M. van der Wielen, Sreekanth Chilamkurthi, Marc Noverraz
Publikováno v:
Journal of Chromatography A. 1217:6829-6850
The thermodynamic modeling of protein adsorption on mixed-mode adsorbents functionalized with ligands carrying both hydrophobic and electrostatic groups was undertaken. The developed mixed mode isotherm was fitted with protein adsorption data obtaine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8508be79c7b889d063cd22259b4412c7
https://doi.org/10.1016/j.chroma.2010.07.069
https://doi.org/10.1016/j.chroma.2010.07.069
Autor:
Jack T. Pronk, Jan-Maarten A. Geertman, Antonius J. A. van Maris, Wouter A. van Winden, Dick Schipper, Cor Ras, Joseph J. Heijnen, Roelco J. Kleijn, Beckley K. Nfor
Publikováno v:
FEMS Yeast Research. 7:216-231
This study focuses on unravelling the carbon and redox metabolism of a previously developed glycerol-overproducing Saccharomyces cerevisiae strain with deletions in the structural genes encoding triosephosphate isomerase ( TPI1 ), the external mitoch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a2cc58f35bdc5a44613de87118658e1
https://doi.org/10.1111/j.1567-1364.2006.00180.x
https://doi.org/10.1111/j.1567-1364.2006.00180.x
Publikováno v:
Fluid Phase Equilibria. 241:268-282
Bioseparation techniques exploit the differences of physicochemical or thermodynamic properties between the product and the contaminants. Rapid development of a downstream process, therefore, requires physicochemical and thermodynamic characterizatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::af1fd5e3ddc4278da10ecb8dc3fa2943
https://doi.org/10.1016/j.fluid.2005.12.011
https://doi.org/10.1016/j.fluid.2005.12.011
Publikováno v:
Pharmaceutical Sciences Encyclopedia
The objective of this chapter is to present a comprehensive overview of the key aspects of downstream process (DSP) development of proteins and peptides. The chapter opens with a general description of the DSP steps and highlights their objectives an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::345e0370b04a69a7a4ad875724e9fc3e
https://doi.org/10.1002/9780470571224.pse507
https://doi.org/10.1002/9780470571224.pse507
Autor:
Michel H.M. Eppink, Peter Verhaert, Marcel Ottens, Tangir Ahamed, Luuk A.M. van der Wielen, Beckley K. Nfor, Gijs W.K. van Dedem, Emile J.A.X. van de Sandt
Publikováno v:
Chemical Engineering Science, 89, 185-195
Chemical Engineering Science 89 (2013)
Chemical Engineering Science 89 (2013)
A model-based rational protein purification process synthesis methodology that addresses the challenge of selecting the most optimal process scheme from several possible alternatives is presented in this study. The main rationale behind the methodolo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec05cba12340ddd95fbd64be24e8ee9d
https://research.wur.nl/en/publications/model-based-rational-methodology-for-protein-purification-process
https://research.wur.nl/en/publications/model-based-rational-methodology-for-protein-purification-process
Autor:
Diego S. Zuluaga, Beckley K. Nfor, and Marcel Ottens, Peter Verhaert, Luuk A.M. van der Wielen, Peter J.T. Verheijen
Publikováno v:
Biotechnology progress. 27(6)
A model-based rational strategy for the selection of chromatographic resins is presented. The main question being addressed is that of selecting the most optimal chromatographic resin from a few promising alternatives. The methodology starts with chr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::588396d53240a5be1bbba2d4d6dd0f50
https://pubmed.ncbi.nlm.nih.gov/22238769
https://pubmed.ncbi.nlm.nih.gov/22238769
Autor:
Marcel Ottens, Peter Verhaert, Martijn W. H. Pinkse, Michel H.M. Eppink, Gijs W.K. van Dedem, Emile J.A.X. van de Sandt, Beckley K. Nfor, Tangir Ahamed, Luuk A.M. van der Wielen
Publikováno v:
Biotechnology and Bioengineering 109 (2012) 12
Biotechnology and Bioengineering, 109(12), 3070-3083
Biotechnology and Bioengineering, 109(12), 3070-3083
A multi-dimensional fractionation and characterization scheme was developed for fast acquisition of the relevant molecular properties for protein separation from crude biological feedstocks by ion-exchange chromatography (IEX), hydrophobic interactio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::717c3ed0a2bcc7dcec7aef34adc53979
https://pubmed.ncbi.nlm.nih.gov/22688729
https://pubmed.ncbi.nlm.nih.gov/22688729
Autor:
Koenraad R. Wiedhaup, Peter Verhaert, Marcel Ottens, Beckley K. Nfor, Luuk A.M. van der Wielen, Nienke N. Hylkema
Publikováno v:
Journal of chromatography. A. 1218(49)
Salt-induced protein precipitation and hydrophobic interaction chromatography (HIC) are two widely used methods for protein purification. In this study, salt effects in protein precipitation and HIC were investigated for a broad combination of protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14d2e0032e82f9639c8ac86e9fb2bd88
https://pubmed.ncbi.nlm.nih.gov/21868020
https://pubmed.ncbi.nlm.nih.gov/21868020