Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules

Autor: Helena Schulz-Mirbach, Jan Lukas Krüsemann, Theofania Andreadaki, Jana Natalie Nerlich, Eleni Mavrothalassiti, Simon Boecker, Philipp Schneider, Moritz Weresow, Omar Abdelwahab, Nicole Paczia, Beau Dronsella, Tobias J. Erb, Arren Bar-Even, Steffen Klamt, Steffen N. Lindner

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)

Abstract Anaerobic microbial fermentations provide high product yields and are a cornerstone of industrial bio-based processes. However, the need for redox balancing limits the array of fermentable substrate-product combinations. To overcome this lim

Externí odkaz: https://doaj.org/article/70455d7d634d43588ef2f556c5b5c224

Activating Silent Glycolysis Bypasses in Escherichia coli

Autor: Camillo Iacometti, Katharina Marx, Maria Hönick, Viktoria Biletskaia, Helena Schulz-Mirbach, Beau Dronsella, Ari Satanowski, Valérie A. Delmas, Anne Berger, Ivan Dubois, Madeleine Bouzon, Volker Döring, Elad Noor, Arren Bar-Even, Steffen N. Lindner

Publikováno v: BioDesign Research, Vol 2022 (2022)

All living organisms share similar reactions within their central metabolism to provide precursors for all essential building blocks and reducing power. To identify whether alternative metabolic routes of glycolysis can operate in E. coli, we complem

Externí odkaz: https://doaj.org/article/fb635e6a310749259efc808565dd22a6

Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation

Autor: Justine Turlin, Beau Dronsella, Alberto De Maria, Steffen N. Lindner, Pablo I. Nikel

Publikováno v: Turlin, J, Dronsella, B, De Maria, A, Lindner, S N & Nikel, P I 2022, ' Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation ', Metabolic Engineering, vol. 74, pp. 191-205 . https://doi.org/10.1016/j.ymben.2022.10.008

Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently produced from CO2—but formatotrophy has been engineered in only a few industrially-relevant microbial hosts. We addressed the challenge of expanding the fe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80b3bf1358cf50d550a3a8ddac5eec16
https://doi.org/10.1016/j.ymben.2022.10.008

Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation

Autor: Nico J, Claassens, Ari, Satanowski, Viswanada R, Bysani, Beau, Dronsella, Enrico, Orsi, Vittorio, Rainaldi, Suzan, Yilmaz, Sebastian, Wenk, Steffen N, Lindner

Publikováno v: Advances in biochemical engineering/biotechnology. 180

In recent years the reductive glycine pathway (rGlyP) has emerged as a promising pathway for the assimilation of formate and other sustainable C1-feedstocks for future biotechnology. It was originally proposed as an attractive "synthetic pathway" to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::85593ff2781ac58d5c63e369c8aea50e
https://pubmed.ncbi.nlm.nih.gov/35364693

Activating silent glycolysis bypasses inEscherichia coli

Autor: H. Schulz-Mirbach, Elad Noor, Beau Dronsella, V. A. Delmas, K. Marx, Anne Berger, Ivan Dubois, Arren Bar-Even, Ari Satanowski, Madeleine Bouzon, V. Doering, Steffen N. Lindner, V. Biletskaia, C. Iacometti, M. Hoenick

All living organisms share similar reactions within their central metabolism to provide precursors for all essential building blocks and reducing power. To identify whether alternative metabolic routes of glycolysis can operate inE. coli, we compleme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d392f8feea9471de98d7fb7ec26e31e3
https://doi.org/10.1101/2021.11.18.468982