Výsledky vyhledávání - "Beatrice S. Schläpfer"

Development of optimized transfectoma cell lines for production of chimeric antibodies in hollow fiber cell culture systems

Autor: Gerd Pluschke, Anke-Peggy Holtorf, Marcel Scheibler, Beatrice S. Schläpfer, Hai Van Nguyen

Publikováno v: Biotechnology and Bioengineering. 45:310-319

Methods for the selection of transfectoma cells that express large quantities of mouse-human chimeric antibodies have been develped. SP2/0 mouse myeloma cells were transfected with pSV2-gpt and pSV2-neo based immunoglobulin expression vectors. Double

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aac973332b64929dbda27929c0cef568
https://doi.org/10.1002/bit.260450405

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Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacillus stearothermophilus

Autor: Beatrice S. Schläpfer, Herbert Zuber

Publikováno v: Gene. 122:53-62

The structural genes encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and the N-terminal part of triosephosphate isomerase (TIM) from mesophilic Bacillus megaterium DSM319 have been cloned as a gene cluster (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5238fbc5e0388289775d826ed213e5d
https://doi.org/10.1016/0378-1119(92)90031-j

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Generation of chimeric monoclonal antibodies from mice that carry human immunoglobulin Cgamma1 heavy of Ckappa light chain gene segments

Autor: Markus Zwickl, Oscar Kashala, Jutta Marfurt, Gilles Sansig, Andrea Joss, Norman Hardman, Gerd Pluschke, Aribert Stief, Susanne Linkert, Beatrice S. Schläpfer, Claudia Daubenberger, Markus Schröder, Herman van der Putten

Publikováno v: Journal of immunological methods. 215(1-2)

Gene targeting in mouse embryonic stem (ES) cells was used to replace (i) the mouse immunoglobulin heavy chain (IgH) Cgamma2a gene segment (mCgamma2a) with the human Cgamma1 gene segment (hCgamma1), and (ii) the mouse immunoglobulin light chain (IgL)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73f8ad8354b1bf67065e2a0fc7ad6897
https://pubmed.ncbi.nlm.nih.gov/9744745

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Correlation between secreted and membrane-bound IgG in mouse myeloma cells transfected with chimeric immunoglobulin heavy and light chain genes

Autor: Monique Ducarre, Beatrice S. Schläpfer, Gerd Pluschke, Josef Brüggen

Publikováno v: Biotechnology and bioengineering. 49(4)

Mouse myeloma cells were transfected with pSV2-gpt and pSV2-neo based immunoglobulin expression vectors. Double transfectants were selected using the xanthine-guanine phosphoribosyl transferase (gpt) and the neomycin (neo) selection marker genes. A b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2dc4ed401deb3c5db46a1fa92186465
https://pubmed.ncbi.nlm.nih.gov/18623602

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