Cas9-expressing chickens and pigs as resources for genome editing in livestock

Autor: Daniel Wise, Harald Luksch, Lucie Carrier, James C. Kaufman, Lutz Kettler, Nina Simm, Tatiana Flisikowska, Tarik Bozoglu, Carolin Perleberg, Benedikt B. Kaufer, Denise Duda, Valeri Zakhartchenko, Hicham Sid, Antonina Schlickenrieder, Mayuko Kurome, Angelika Schnieke, Eckhard Wolf, Beate Rieblinger, Christian Kupatt, Ibrahim T. Hagag, Alessandro Grodziecki, Barbara Kessler, Kamila Lengyel, Krzysztof Flisikowski, Benjamin Schusser, Andrea Bähr, Romina Klinger

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance Genetically modified animals are crucial for functional studies and translational biomedical research. However, the generation of genome-edited large animals is time consuming and inefficient. Here, we report the generation of transgenic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd665fa38437cd699462606eb34d756a
http://europepmc.org/articles/PMC7958376

Porcine model elucidates function of p53 isoform in carcinogenesis

Autor: Beate Rieblinger, Isabel Hellmuth, Hubert Pausch, Benjamin Schade, Dieter Saur, Katja Steiger, Tatiana Flisikowska, Eva Kappe, Konrad Fischer, Alexander Kind, Bernhard Klinger, Jean-Christophe Bourdon, Reiner Burgkart, Angelika Schnieke, Krzysztof Flisikowski, Guanglin Niu, Alexander Carrapeiro, Brigitte Böhm

BackgroundThe pig has long been an important animal species for biomedical research. Recent years has also seen an increasing number of genetically engineered pig models of human diseases including cancer. We previously generated pigs with a modified

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4c2ca1fd25ee435afd069c08250fae8b
https://doi.org/10.1101/2020.09.07.286005

Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2

Autor: Robert Rieben, Wei Liang, Beate Rieblinger, Angelika Schnieke, Reinhard Schwinzer, Eckhard Wolf, Julia Zuber, Riccardo Sfriso, Konrad Fischer, Alexander Kind, Mayuko Kurome, Bernhard Klinger, Andrea Fischer, Valeri Zakhartchenko, Rabea Hein, Barbara Kessler, Krzysztof Flisikowski

Publikováno v: Fischer, Konrad; Rieblinger, Beate; Hein, Rabea; Sfriso, Riccardo; Zuber, Julia; Fischer, Andrea; Klinger, Bernhard; Liang, Wei; Flisikowski, Krzysztof; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Wolf, Eckhard; Rieben, Robert; Schwinzer, Reinhard; Kind, Alexander; Schnieke, Angelika (2020). Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2. Xenotransplantation, 27(1), e12560. Wiley 10.1111/xen.12560

BACKGROUND Cell surface carbohydrate antigens play a major role in the rejection of porcine xenografts. The most important for human recipients are α-1,3 Gal (Galactose-alpha-1,3-galactose) causing hyperacute rejection, also Neu5Gc (N-glycolylneuram

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf4900f705a96e01d2a8495ef0b56e62
https://boris.unibe.ch/136305/1/Fischer_et_al-2019-Xenotransplantation.pdf

Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing

Autor: Valeri Zakhartchenko, Tatiana Flisikowska, Eckhard Wolf, Marek Switonski, Reinhard Schwinzer, Beate Rieblinger, Alexander Kind, Krzysztof Flisikowski, Joachim Denner, Konrad Fischer, Simone Kraner-Scheiber, Izabela Szczerbal, Wiebke Baars, Mayuko Kurome, Anna Buermann, Susanne Christan, Elena Plotzki, Heiner Niemann, Barbara Kessler, Angelika Schnieke, Marlene Edlinger, Björn Petersen

Publikováno v: Scientific Reports

Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection and acute vascular rejection mechanisms mounted by the recipient. The challenge is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e3634b68c6a5a91cd43135bf73883ca