Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Beate, Kaufmann"'
Autor:
Nanami Kikuchi, Sarah Goldberg, Beate Kaufmann, Lisa Kermas, Roee Amit, Or Willinger, Noa Navon
Publikováno v:
ACS Synthetic Biology. 10:1808-1820
We present Triplex-seq, a deep-sequencing method that systematically maps the interaction space between an oligo library of ssDNA triplex-forming oligos (TFOs) and a particular dsDNA triplex target site (TTS). We demonstrate the method using a random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3913f37af0dea5e3c35c66d8c7dee826
https://doi.org/10.1021/acssynbio.1c00122
https://doi.org/10.1021/acssynbio.1c00122
Autor:
Beate, Kaufmann, Or, Willinger, Nanami, Kikuchi, Noa, Navon, Lisa, Kermas, Sarah, Goldberg, Roee, Amit
Publikováno v:
ACS synthetic biology. 10(8)
We present Triplex-seq, a deep-sequencing method that systematically maps the interaction space between an oligo library of ssDNA triplex-forming oligos (TFOs) and a particular dsDNA triplex target site (TTS). We demonstrate the method using a random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::440254d8e0ee99de38d0e6aa08de45ee
https://pubmed.ncbi.nlm.nih.gov/34374529
https://pubmed.ncbi.nlm.nih.gov/34374529
Autor:
Asif Gil, Heidi Leonard, Inbal Adir, Tal Fried, Nofar Shasha, Ofek Elul, Shilo Ohayon, Bar Mayo, Michal Brunwasser-Meirom, Beate Kaufmann, Sharbel Zahran, Yuri Haimov, Shani Weiner, Noa Katz, Shiran Sarig, Roee Amit, Tzila Davidov, Ester Segal, Shirane Tsedef, Naor Granik, Alexandra Ereskovsky
Publikováno v:
ACS biomaterials scienceengineering. 5(2)
Whole cell bioreporters, such as bacterial cells, can be used for environmental and clinical sensing of specific analytes. However, the current methods implemented to observe such bioreporters in the form of chemotactic responses heavily rely on micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e3a881ab426beb53c1106b473055d42
https://pubmed.ncbi.nlm.nih.gov/33405824
https://pubmed.ncbi.nlm.nih.gov/33405824
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Autor:
Zohar Yakhini, Lisa Kermas, Oz Solomon, Roee Amit, Leon Anavy, Sarah Goldberg, Noa Eden, Orna Atar, Beate Kaufmann, Or Willinger
SummaryNature provides a rich toolbox of dynamic nucleic acid structures that are widespread in cells and affect multiple biological processes1. Recently, non-canonical structures gained renewed scientific and biotechnological interest2,3. One partic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75f2d26d9539fe59f09da61faf312fce
Autor:
Roee Amit, Noa Katz, Beate Kaufmann, Orna Atar, Sarah Goldberg, Roni Cohen, Zohar Yakhini, Oz Solomon
Publikováno v:
ACS synthetic biology. 7(12)
We study translation repression in bacteria by engineering a regulatory circuit that functions as a binding assay for RNA binding proteins (RBP) in vivo. We do so by inducing expression of a fluorescent protein-RBP chimera, together with encoding its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::522b9b7ae0130eb8b3d18bf44a59c4ab
https://pubmed.ncbi.nlm.nih.gov/30408420
https://pubmed.ncbi.nlm.nih.gov/30408420
Autor:
Roee Amit, Oz Solomon, Noa Katz, Sarah Goldberg, Orna Atar, Beate Kaufmann, Zohar Yakhini, Roni Cohen
Publikováno v:
Cell systems. 9(1)
Summary The construction of complex gene-regulatory networks requires both inhibitory and upregulatory modules. However, the vast majority of RNA-based regulatory “parts” are inhibitory. Using a synthetic biology approach combined with SHAPE-seq,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ffbb1cc314e3509d0aaa1a66cdd7ec
https://pubmed.ncbi.nlm.nih.gov/31129060
https://pubmed.ncbi.nlm.nih.gov/31129060
Induced Protein-RNA Interactions in the 5’ UTR Lead to Conformational Changes that Alter Translation
Autor:
Orna Atar, Roni Cohen, Beate Kaufmann, Zohar Yakhini, Roee Amit, Sarah Goldberg, Noa Katz, Oz Solomon
Publikováno v:
SSRN Electronic Journal.
We study translational regulation by a 5’ UTR sequence encoding the binding site of an RNA-binding protein (RBP) in bacteria, using a reporter assay and selective 2′-hydroxyl acylation analysed by primer extension sequencing (SHAPE-Seq). We teste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9ea8179904d709d624dd6b12b6881a53
https://doi.org/10.2139/ssrn.3263636
https://doi.org/10.2139/ssrn.3263636
Autor:
Anne Müller, Philipp M Warmer, Fenja Gawlas, Philipp Schwenk, Michael Agne, Alica J. Emhardt, Lisa J Schmunk, Manuel C. Scheidmann, Gerald Radziwill, Thomas J Juretschke, Patrick Gonschorek, Maximilian R Stammnitz, Stefan D Krämer, Beate Kaufmann, Lisa M Schäfer, Nadine Gillich, Ilona Blank, Alina Rudorf, Natalie Louis, Christoph G Gäbelein, Wilfried Weber, Adrian Fischer, Hanna J. Wagner
Publikováno v:
ACS Synthetic Biology. 3:986-989
The ability to control mammalian genes in a synergistic mode using synthetic transcription factors is highly desirable in fields of tissue engineering, stem cell reprogramming and fundamental research. In this study, we developed a standardized toolk
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7817e6dd7b64fbbc18cae903e113da8d
https://doi.org/10.1021/sb500035y
https://doi.org/10.1021/sb500035y
Autor:
Noa Katz, Roni Cohen, Oz Solomon, Beate Kaufmann, Noa Eden, Orna Atar, Zohar Yakhini, Sarah Goldberg, Roee Amit
We employ a reporter assay and Selective 2′-hydroxyl acylation analysed by primer extension sequencing (SHAPE-seq) to study translational regulation by RNA-binding proteins, in bacteria. We designed 82 constructs, each with a single hairpin based o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a359134459ca2d994702705dda24160