Identification of optimal reference genes for gene expression studies in a focal cerebral ischaemia model - spatiotemporal effects

Autor: Bartosz Pomierny, Weronika Krzyzanowska, Jakub Jurczyk, Beata Strach, Alicja Skorkowska, Innesa Leonovich, Bogusława Budziszewska, Joanna Pera

A proper reference gene (RG) is required to reliably measure mRNA levels in biological samples via quantitative reverse transcription PCR (RT-qPCR). Various experimental paradigms require specific and stable RGs. In studies using rodent models of bra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33a4f6ef08d4c962a808f5aa5c7ed5b2
https://ruj.uj.edu.pl/xmlui/handle/item/293661

Thermo- and pH-Responsible Gels for Efficient Protein Adsorption and Desorption

Autor: Izabela Poplewska, Beata Strachota, Adam Strachota, Grzegorz Poplewski, Dorota Antos

Publikováno v: Molecules, Vol 29, Iss 20, p 4858 (2024)

Protein adsorption behavior was examined on poly(N-isopropylacrylamide-co-sodium methacrylate)-based hydrogels at different temperatures: 5, 20, and 37 °C, and pH: 4.5, 7, and 9.2. The hydrogels, whose covalent skeleton contains pendant anionic unit

Externí odkaz: https://doaj.org/article/531c73e65bd047138229c97352ff867a

In vitro and in vivo behavior of ground tadalafil hot-melt extrudates: How the carrier material can effectively assure rapid or controlled drug release

Autor: Andrzej Wróbel, Renata Jachowicz, Beata Strach, Anna Krupa, Juergen Siepmann, Oriane Cantin, Zbisław Tabor, Elżbieta Wyska

Publikováno v: International journal of pharmaceutics. 528(1-2)

Different types of ground hot-melt extrudates loaded with 10, 20 or 30 % of the poorly water-soluble drug tadalafil were prepared and characterized in vitro and in vivo (in rats). Soluplus was used as an amorphous carrier material, whereas mannitol a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a5aa9e48789743ac2597bd075db10fe
https://pubmed.ncbi.nlm.nih.gov/28559217

Synthesis and Morphology Characteristics of New Highly Branched Polycaprolactone PCL

Autor: Aleksandra Zioło, Beata Mossety-Leszczak, Małgorzata Walczak, Beata Strachota, Adam Strachota, Kamil Awsiuk, Natalia Janiszewska, Joanna Raczkowska

Publikováno v: Molecules, Vol 29, Iss 5, p 991 (2024)

A simple and efficient method for the synthesis of biodegradable, highly branched polycaprolactone (PCL) is presented. The solvent-free (bulk) reaction was carried out via ring opening polymerization (ROP), catalyzed by tin octanoate Sn(Oct)2, and it

Externí odkaz: https://doaj.org/article/9fcc3a70dc1f45aaa926757fbc3753a7

High-Energy Ball Milling as Green Process To Vitrify Tadalafil and Improve Bioavailability

Autor: Jean-François willart, Renata Jachowicz, Beata Strach, Anna Krupa, Elżbieta Wyska, Marc Descamps, Florence Danede

Publikováno v: Molecular Pharmaceutics
Molecular Pharmaceutics, American Chemical Society, 2016, 13 (11), pp.3891-3902. ⟨10.1021/acs.molpharmaceut.6b00688⟩
Molecular Pharmaceutics, 2016, 13 (11), pp.3891-3902. ⟨10.1021/acs.molpharmaceut.6b00688⟩

In this study, the suitability of high-energy ball milling was investigated with the aim to vitrify tadalafil (TD) and improve its bioavailability. To achieve this goal, pure TD as well as binary mixtures composed of the drug and Soluplus (SL) were c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68a6896466bd0b10f67ec581778987b7
https://ruj.uj.edu.pl/xmlui/handle/item/99586