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Helicobacter pylori is an important human pathogen which causes both gastric and duodenal ulcers and is associated with gastric cancer and lymphoma. This microorganism synthesizes fucosylated oligosaccharides, predominantly the Galb-1,4GlcNAc (Type I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70632149457e504bac85292575f607d7
http://doc.rero.ch/record/300549/files/cwj004.pdf
http://doc.rero.ch/record/300549/files/cwj004.pdf
Publikováno v:
European Journal of Biochemistry. 268:5930-5936
NMR studies of amyloid beta-peptides (A beta) in aqueous solution provide a novel way in which to characterize the apparent Alzheimer's disease-related conformational polymorphism of A beta. In the aqueous medium, neither of the polypeptides A beta(1
Publikováno v:
Cytotechnology. 35:157-164
We describe a generic plasmid purification process for producing DNA for larger-scale transient transfection. Data on plasmid quality with regard to residual protein, endotoxin content and presence of different plasmid forms is given. The effects of
Autor:
Beat Wipf, Brigitte D'Arcy, Allan D'Arcy, Christian Oefner, Chantana Yuvaniyama, Glenn E. Dale
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 56:894-897
Neutral endopeptidase (NEP) is a mammalian zinc metalloprotease involved in the inactivation of a wide variety of regulatory peptides such as enkephalins and atrial natiuretic factor. The soluble extracellular domain of NEP (sNEP) was expressed in th
Autor:
Christopher Gray, Hanno Langen, Beat Wipf, Michael Fountoulakis, Bela Takacs, Peter Berndt, Stefan Evers, Hans-Werner Lahm
Publikováno v:
Electrophoresis. 21:411-429
We have constructed a two-dimensional database of the proteome of Haemophilus influenzae, a bacterium of medical interest of which the complete genome, comprising about 1742 open reading frames, has been sequenced. The soluble protein fraction of the
Publikováno v:
Journal of Biomolecular NMR. 11:279-288
An HMQC experiment is proposed, dubbed FHMQC, where water flip-back is achieved by a single water-selective pulse preceding the basic HMQC pulse sequence. The scheme is demonstrated with a 15N, 1H-HMQC spectrum of uniformly 15N/2H-labelled S. aureus
Publikováno v:
Journal of Molecular Biology. 274:661-675
The solution structure of recombinant human interferon alpha-2a (Roferon-A) has been determined by multidimensional heteronuclear NMR spectroscopy. The calculations using simulated annealing produced a family of 24 convergent structures which satisfy
Autor:
Peter Hartman, Rudolf L. Then, Clemens Broger, Hanno Langen, Malcolm G. P. Page, R. Dehoogt, Hans Locher, Allan D'Arcy, Beat Wipf, Glenn E. Dale, Synese Jolidon, A. M. Labhardt, Christian Oefner, Dietrich Stüber, Ivan Kompis
Publikováno v:
Journal of Molecular Biology. 266:23-30
A single amino acid substitution, Phe98 to Tyr98, in dihydrofolate reductase (DHFR) is the molecular origin of trimethoprim (TMP) resistance in Staphylococcus aureus. This active site amino acid substitution was found in all S. aureus TMP-resistant c
Autor:
Beat Wipf, Heinz Döbeli, Martin Zulauf, Nicholas Draeger, Bernd Seilheimer, Peter Jakob, Dieter Schmidt, Dietrich Stüber, Gerda Huber
Publikováno v:
Nature Biotechnology. 13:988-993
Senile plaques, a neuropathological hallmark of Alzheimer's disease, consist primarily of insoluble aggregates of beta-amyloid peptide (A beta). A 42-residue peptide (A beta 1-42) appears to be the predominant form. In contrast to A beta 1-40, A beta
Autor:
André Strauss, Guido Vogel, Claudia Bardouille, Georg Schmid, Christian Miscenic, Valentin Pflüger, Sylvie Antz, Bernard Jenni, Beat Wipf, Dominik Ziegler, Eric Dumermuth
Publikováno v:
BMC Proceedings
BMC Proceedings, Vol 5, Iss Suppl 8, p P45 (2011)
BMC Proceedings, Vol 5, Iss Suppl 8, p P45 (2011)
Misidentification or cross-contamination of cell lines used in biotechnology or diagnostic settings are a challenge for laboratories and cell culture repositories. Masters et al. [1] among others reported the occurrence of large numbers of unrecogniz