Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols.

Autor: Bean BDM; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada., Mulvihill CJ; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Garge RK; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Boutz DR; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA.; DEVCOM Army Research Laboratory-South, Austin, 78712, TX, USA., Rousseau O; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada., Floyd BM; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Cheney W; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada., Gardner EC; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Ellington AD; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Marcotte EM; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA., Gollihar JD; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX, 78712, USA. jgollihar2@houstonmethodist.org.; DEVCOM Army Research Laboratory-South, Austin, 78712, TX, USA. jgollihar2@houstonmethodist.org.; Center for Molecular and Translational Human Infectious Diseases Research, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX, USA. jgollihar2@houstonmethodist.org., Whiteway M; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada., Martin VJJ; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada. vincent.martin@concordia.ca.

Publikováno v: Nature communications [Nat Commun] 2022 May 24; Vol. 13 (1), pp. 2882. Date of Electronic Publication: 2022 May 24.

Competitive organelle-specific adaptors recruit Vps13 to membrane contact sites.

Autor: Bean BDM; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.; Department of Medical Genetics, University of British Columbia, Vancouver, Canada., Dziurdzik SK; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.; Department of Medical Genetics, University of British Columbia, Vancouver, Canada., Kolehmainen KL; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada., Fowler CMS; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada., Kwong WK; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada., Grad LI; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.; Department of Medical Genetics, University of British Columbia, Vancouver, Canada., Davey M; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada., Schluter C; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada., Conibear E; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada conibear@cmmt.ubc.ca.; Department of Medical Genetics, University of British Columbia, Vancouver, Canada.

Publikováno v: The Journal of cell biology [J Cell Biol] 2018 Oct 01; Vol. 217 (10), pp. 3593-3607. Date of Electronic Publication: 2018 Jul 17.

Quantitative high-content imaging identifies novel regulators of Neo1 trafficking at endosomes.

Autor: Dalton LE; Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of British Columbia, Vancouver, BC V6T 1Z3, Canada., Bean BDM; Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of British Columbia, Vancouver, BC V6T 1Z3, Canada., Davey M; Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC V5Z 4H4, Canada., Conibear E; Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC V5Z 4H4, Canada conibear@cmmt.ubc.ca.; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.; Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

Publikováno v: Molecular biology of the cell [Mol Biol Cell] 2017 Jun 01; Vol. 28 (11), pp. 1539-1550. Date of Electronic Publication: 2017 Apr 12.