The methyl phosphate capping enzyme Bin3 is a stable component of the fission yeast telomerase holoenzyme

Autor: Porat J, Moaine El Baidouri, Bayfield Ma, Jean-Marc Deragon, Grigull J

The telomerase holoenzyme is critical for maintaining eukaryotic genome integrity. In addition to a reverse transcriptase and an RNA template, telomerase contains additional proteins that protect the telomerase RNA and promote holoenzyme assembly. He

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https://hal.archives-ouvertes.fr/hal-03439286/document

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Canada's contributions to RNA research: past, present, and future perspectives.

Autor: Lécuyer E; Institut de Recherches Cliniques de Montréal (IRCM), Montréal, QC, Canada.; Département de Biochimie et de Médecine Moléculaire, Université de Montréal, Montréal, QC, Canada.; Division of Experimental Medicine, McGill University, Montréal, QC, Canada., Sauvageau M; Institut de Recherches Cliniques de Montréal (IRCM), Montréal, QC, Canada.; Département de Biochimie et de Médecine Moléculaire, Université de Montréal, Montréal, QC, Canada.; Department of Biochemistry, McGill University, Montréal, QC, Canada., Kothe U; Department of Chemistry, University of Manitoba, Winnipeg, MB, Canada., Unrau PJ; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada., Damha MJ; Department of Chemistry, McGill University, Montréal, QC, Canada., Perreault J; Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Laval, QC, Canada., Abou Elela S; Département de Microbiologie et Infectiologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Bayfield MA; Department of Biology, York University, Toronto, ON, Canada., Claycomb JM; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada., Scott MS; Département de Biochimie et de Génomique Fonctionnelle, Université de Sherbrooke, Sherbrooke, QC, Canada.

Publikováno v: Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.

Electrophoretic mobility shift assays (EMSAs) for in vitro detection of protein-nucleic acid interactions.

Autor: Mansouri-Noori F; Department of Biology, York University, Toronto, ON M3J 1P3, Canada. Electronic address: farnaz.mansouri@gmail.com., Bayfield MA; Department of Biology, York University, Toronto, ON M3J 1P3, Canada. Electronic address: bayfield@yorku.ca.

Publikováno v: STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103128. Date of Electronic Publication: 2024 Jun 13.