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The spatial distributions of pre-mRNAs suggest post-transcriptional splicing of specific introns within endogenous genes

Autor: Coté, Allison, Coté, Chris, Bayatpour, Sareh, Drexler, Heather L., Alexander, Katherine A., Chen, Fei, Wassie, Asmamaw T., Boyden, Edward S., Berger, Shelley, Churchman, L. Stirling, Raj, Arjun

Splicing is the molecular process by which introns are removed from pre-mRNA and exons are joined together to form the sequence of the mature mRNA. Measuring the timing of splicing relative to the transcription of nascent RNA has yielded conflicting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=sharebioRxiv::b6e5a4b6427e97a281ef3cee7e76d4c6

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ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.

Autor: Rouhanifard SH; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Mellis IA; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.; Genomics and Computational Biology Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Dunagin M; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Bayatpour S; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Jiang CL; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.; Cell and Molecular Biology Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Dardani I; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Symmons O; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Emert B; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.; Genomics and Computational Biology Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Torre E; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Cote A; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA., Sullivan A; Altius Institute for Biomedical Sciences, Seattle, Washington, USA., Stamatoyannopoulos JA; Altius Institute for Biomedical Sciences, Seattle, Washington, USA., Raj A; Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.; Genomics and Computational Biology Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2018 Nov 12. Date of Electronic Publication: 2018 Nov 12.