Zobrazeno 1 - 10 of 13 pro vyhledávání: '"Baubek Spanov"'
1
Akademický článek
Analytical tools for the characterization of deamidation in monoclonal antibodies
Autor: Baubek Spanov, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Publikováno v: Journal of Chromatography Open, Vol 2, Iss , Pp 100025- (2022)
This review focuses on the deamidation of asparagine in monoclonal antibodies, one of the well-known degradation pathways. The importance of close monitoring of deamidation from early developability assessment to in vivo studies is discussed conseque
Externí odkaz: https://doaj.org/article/4a5907208d3948cfb9941b6320065cf8
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2
Akademický článek
Effect of Trastuzumab–HER2 Complex Formation on Stress-Induced Modifications in the CDRs of Trastuzumab
Autor: Baubek Spanov, Victoria Aboagye, Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Publikováno v: Frontiers in Chemistry, Vol 9 (2022)
Asparagine deamidation and aspartic acid isomerization in the complementarity determining regions (CDRs) of monoclonal antibodies may alter their affinity to the target antigen. Trastuzumab has two hot spots for deamidation and one position for isome
Externí odkaz: https://doaj.org/article/4c384339d848482892f04d0f70870428
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5
Biotransformation of Trastuzumab and Pertuzumab in Breast Cancer Patients assessed by Affinity Enrichment and Ion Exchange Chromatography
Autor: Oladapo Olaleye, Baubek Spanov, Peter Bults, Anna van der Voort, Natalia Govorukhina, Gabe S. Sonke, Peter Horvatovich, Nico C. van de Merbel, Rainer Bischoff
Publikováno v: Drug Metabolism and Disposition, 50(12), 249-256. AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
Therapeutic proteins (TPs) are known to be heterogeneous due to modifications that occur during the production process and storage. Modifications may also occur in TPs after their administration to patients due to in vivo biotransformation. Ligand bi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae87696c75e933ab48fb7eec5070321d
https://research.rug.nl/en/publications/de84de11-0a42-40fd-b01c-be0b04d60f7e
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7
Revealing charge heterogeneity of stressed trastuzumab at the subunit level
Autor: Baubek Spanov, Bas Baartmans, Oladapo Olaleye, Simone Nicolardi, Natalia Govorukhina, Manfred Wuhrer, Nico C. van de Merbel, Rainer Bischoff
Publikováno v: Analytical and Bioanalytical Chemistry, 415(8), 1505-1513. SPRINGER HEIDELBERG
Trastuzumab is known to be heterogeneous in terms of charge. Stressing trastuzumab under physiological conditions (pH 7.4 and 37 °C) increases charge heterogeneity further. Separation of charge variants of stressed trastuzumab at the intact protein
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4fb47a70166e8bd706c21d7627479d9
https://hdl.handle.net/1887/3590794
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8
Bioanalytical methodology to study the in vivo biotransformation of therapeutic proteins
Autor: Baubek Spanov
Publikováno v: University of Groningen
Therapeutic proteins (TPs) have emerged as an important class of pharmaceuticals for the treatment of various severe diseases. TPs are highly specific and present fewer unwanted side effects than traditional small molecules. A variety of chemical and
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::2b80b6799418e15fad553f8db3c3b316
https://research.rug.nl/en/publications/2b7204eb-5126-44aa-9230-f6c657ffda55
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9
Intact protein bioanalysis by liquid chromatography – High-resolution mass spectrometry
Autor: Peter Bults, Nico C van de Merbel, Rainer Bischoff, Oladapo Olaleye, Baubek Spanov
Publikováno v: Journal of Chromatography B
This review discusses the challenges of quantitative protein bioanalysis by LC-MS at the protein level. We will notably address the possibilities and current limitations of protein sample preparation, separation by LC, the challenge of interpreting p
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c415474d98b728d93a646cb1c3b6f3e1
https://doi.org/10.1016/j.jchromb.2019.01.032
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10
Change of charge variant composition of trastuzumab upon stressing at physiological conditions
Autor: Natalia Govorukhina, Oladapo Olaleye, Nico C van de Merbel, Baubek Spanov, Gestur Vidarsson, Jos Hermans, Rainer Bischoff, Nico Lingg, Alois Jungbauer, Arthur E. H. Bentlage
Publikováno v: Journal of Chromatography A
Journal of chromatography, 1655:462506. Elsevier
Journal of Chromatography A, 1655:462506. ELSEVIER SCIENCE BV
Cation-exchange chromatography is a widely used approach to study charge heterogeneity of monoclonal antibodies. Heterogeneity may arise both in vitro and in vivo because of the susceptibility of monoclonal antibodies to undergo chemical modification
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2a6274210c9d4c1004805a217126ebd
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