Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury.

Autor: David M Rissin, Barbara López-Longarela, Salvatore Pernagallo, Hugh Ilyine, A D Bastiaan Vliegenthart, James W Dear, Juan J Díaz-Mochón, David C Duffy

Publikováno v: PLoS ONE, Vol 12, Iss 7, p e0179669 (2017)

We have developed a single probe method for detecting microRNA from human serum using single molecule arrays, with sequence specificity down to a single base, and without the use of amplification by polymerases. An abasic peptide nucleic acid (PNA) p

Externí odkaz: https://doaj.org/article/06f0f8682210475f9dba71838795e9f1

Circulating argonaute-bound microRNA-126 reports vascular dysfunction and treatment response in acute and chronic kidney disease

Autor: James W. Dear, Laura Rivoli, Alicja Czopek, David J. Webb, A. D. Bastiaan Vliegenthart, Tariq E. Farrah, Matthew A. Bailey, Kathleen M. Scullion, Neeraj Dhaun, Wilna Oosthuyzen, Robert W. Hunter

Publikováno v: Scullion, K, Vliegenthart, A D B, Rivoli, L, Oosthuyzen, W, Farrah, T, Czopek, A, Webb, D J, Hunter, R W, Bailey, M A & Dhaun, N 2021, ' Circulating argonaute-bound microRNA-126 reports vascular dysfunction and treatment response in acute and chronic kidney disease ', iScience . https://doi.org/10.1016/j.isci.2020.101937
iScience, Vol 24, Iss 1, Pp 101937-(2021)
iScience

Summary Vascular and kidney dysfunction commonly co-exist. There is a need for biomarkers of vascular health. Circulating microRNAs are biomarkers; miR-126 is endothelial cell-enriched. We measured circulating miR-126 in rats with nephrotoxic nephrit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c2a6a3a533a9a90e80ac9566ab895f8
https://doi.org/10.1016/j.isci.2020.101937

Biomarker evaluation of plasma microRNA-122, high-mobility group box-1 and keratin-18 in acute gallstone disease

Autor: James W. Dear, Bastiaan Vliegenthart, Th’ng F, Jonathan D. Lea, Mole Dj, D.J. Antoine

BackgroundA biomarker that stratifies patients with complications of gallstone disease from the denominator pool of people with acute biliary-type symptoms is needed. Circulating microRNA-122 (miRNA-122), high-mobility group box1 (HMGB1), full-length

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01e50b76ec65cce1615ea77ce334cb5f
https://doi.org/10.1101/189589

Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury

Autor: Salvatore Pernagallo, David M. Rissin, Hugh Ilyine, James W. Dear, David C. Duffy, A. D. Bastiaan Vliegenthart, Barbara López-Longarela, Juan J. Díaz-Mochón

Publikováno v: PLoS ONE, Vol 12, Iss 7, p e0179669 (2017)
Rissin, D M, López-Longarela, B, Pernagallo, S, Ilyine, H, Vliegenthart, B, Dear, J, Díaz-Mochón, J J & Duffy, D C 2017, ' Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury ', PLoS ONE . https://doi.org/10.1371/journal.pone.0179669
Digibug. Repositorio Institucional de la Universidad de Granada
instname
PLoS ONE

We have developed a single probe method for detecting microRNA from human serum using single molecule arrays, with sequence specificity down to a single base, and without the use of amplification by polymerases. An abasic peptide nucleic acid (PNA) p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a3fae1820f06172b49aae78c75e6536
http://europepmc.org/articles/PMC5497960?pdf=render

Ethanol consumption produces a small increase in circulating miR-122 in healthy individuals

Autor: David J. Webb, James W. Dear, Noel Sharkey, A. D. Bastiaan Vliegenthart, Jame C McCrae

Publikováno v: McCrae, J C, Sharkey, N, Webb, D J, Vliegenthart, A D B & Dear, J W 2016, ' Ethanol consumption produces a small increase in circulating miR-122 in healthy individuals ', Clinical Toxicology, vol. 54, no. 1, pp. 53-5 . https://doi.org/10.3109/15563650.2015.1112015

INTRODUCTION: MicroRNA 122 (miR-122) is a new circulating biomarker for liver injury, which increases earlier than conventional markers in patients with acetaminophen hepatotoxicity. However, as co-ingestion of ethanol is common with drug overdose, a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc2425a549cb5d7694f081e72c17b10f
https://doi.org/10.3109/15563650.2015.1112015