Zobrazeno 1 - 10
of 167
pro vyhledávání: '"Bashtrykov P"'
Publikováno v:
iScience, Vol 27, Iss 10, Pp 111007- (2024)
Summary: Epigenome editing is an emerging technology that allows to rewrite epigenome states and reprogram gene expression. Here, we have developed allele-specific DNA demethylation editing at gene promoters containing an SNP by sgRNA/dCas9 mediated
Externí odkaz:
https://doaj.org/article/74d6c5a8ed014492b9cef89e3dfc7600
Autor:
Leonie Dossmann, Max Emperle, Michael Dukatz, Alex de Mendoza, Pavel Bashtrykov, Albert Jeltsch
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-12 (2024)
Abstract The DNA methyltransferase DNMT3C appeared as a duplication of the DNMT3B gene in muroids and is required for silencing of young retrotransposons in the male germline. Using specialized assay systems, we investigate the flanking sequence pref
Externí odkaz:
https://doaj.org/article/926964ba06e8461bbcb0aa1dd8e6a35e
Publikováno v:
Mobile DNA, Vol 15, Iss 1, Pp 1-24 (2024)
Abstract Background Repeat elements (REs) play important roles for cell function in health and disease. However, RE enrichment analysis in short-read high-throughput sequencing (HTS) data, such as ChIP-seq, is a challenging task. Results Here, we pre
Externí odkaz:
https://doaj.org/article/6bbedbe0055f4eb0b0b726bf50b4a139
Autor:
Kosuke Yamaguchi, Xiaoying Chen, Brianna Rodgers, Fumihito Miura, Pavel Bashtrykov, Frédéric Bonhomme, Catalina Salinas-Luypaert, Deis Haxholli, Nicole Gutekunst, Bihter Özdemir Aygenli, Laure Ferry, Olivier Kirsh, Marthe Laisné, Andrea Scelfo, Enes Ugur, Paola B. Arimondo, Heinrich Leonhardt, Masato T. Kanemaki, Till Bartke, Daniele Fachinetti, Albert Jeltsch, Takashi Ito, Pierre-Antoine Defossez
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract DNA methylation is an essential epigenetic chromatin modification, and its maintenance in mammals requires the protein UHRF1. It is yet unclear if UHRF1 functions solely by stimulating DNA methylation maintenance by DNMT1, or if it has impor
Externí odkaz:
https://doaj.org/article/f127c2781d354cf983fa3decda8e2001
Autor:
Lisa-Marie Brenner, Florian Meyer, Haiqian Yang, Anja R. Köhler, Pavel Bashtrykov, Ming Guo, Albert Jeltsch, Cristiana Lungu, Monilola A. Olayioye
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-11 (2024)
Abstract Through its involvement in gene transcription and heterochromatin formation, DNA methylation regulates how cells interact with their environment. Nevertheless, the extracellular signaling cues that modulate the distribution of this central c
Externí odkaz:
https://doaj.org/article/bbb4873ca04d44eeab9819190a7f92a3
Autor:
Alexandra G. Kouroukli, Nivethika Rajaram, Pavel Bashtrykov, Helene Kretzmer, Reiner Siebert, Albert Jeltsch, Susanne Bens
Publikováno v:
Clinical Epigenetics, Vol 15, Iss 1, Pp 1-15 (2023)
Abstract Background Activation of dominant oncogenes by small or structural genomic alterations is a common driver mechanism in many cancers. Silencing of such dominantly activated oncogenic alleles, thus, is a promising strategy to treat cancer. Rec
Externí odkaz:
https://doaj.org/article/11af78ae09f44988a6a8dbb6e2a1f3dd
Publikováno v:
Epigenetics & Chromatin, Vol 16, Iss 1, Pp 1-18 (2023)
Abstract Background Epigenome editing refers to the targeted reprogramming of genomic loci using an EpiEditor which may consist of an sgRNA/dCas9 complex that recruits DNMT3A/3L to the target locus. Methylation of the locus can lead to a modulation o
Externí odkaz:
https://doaj.org/article/41d1de7855f14d409bafb99c8a958394
Autor:
Alexandra G. Kouroukli, Anja Fischer, Helene Kretzmer, Emil Chteinberg, Nivethika Rajaram, Selina Glaser, Julia Kolarova, Pavel Bashtrykov, Stephan Mathas, Hans G. Drexler, Hitoshi Ohno, Ole Ammerpohl, Albert Jeltsch, Reiner Siebert, Susanne Bens
Publikováno v:
Blood Cancer Journal, Vol 13, Iss 1, Pp 1-4 (2023)
Externí odkaz:
https://doaj.org/article/a5d245bc7209422783c3ec33c962c0d4
Autor:
Alexander Bröhm, Tabea Schoch, Michael Dukatz, Nora Graf, Franziska Dorscht, Evelin Mantai, Sabrina Adam, Pavel Bashtrykov, Albert Jeltsch
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
DNA methyltransferase 3A (DNMT3A) is shown to efficiently methylate recombinant mononucleosome linker DNA and a role for H3K36me3 in the stimulation of DNA methylation is revealed that is independent of its interaction with the PWWP domain.
Externí odkaz:
https://doaj.org/article/85ca972b3f49401a9ad2f077df84cfcc
Autor:
Sabrina Adam, Julia Bräcker, Viviane Klingel, Bernd Osteresch, Nicole E. Radde, Jens Brockmeyer, Pavel Bashtrykov, Albert Jeltsch
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-13 (2022)
Sabrina Adam et al. use a deep enzymology method to study the effect of neighboring DNA sequence variation on the in vitro activity of Tet1 and Tet2. Their results suggest that flanking sequences could represent an important parameter that influences
Externí odkaz:
https://doaj.org/article/53caa262787a41d4b84543d2f7d4ab38