Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Bas Ponsioen"'
Autor:
Sander Mertens, Maarten A. Huismans, Carla S. Verissimo, Bas Ponsioen, Rene Overmeer, Natalie Proost, Olaf van Tellingen, Marieke van de Ven, Harry Begthel, Sylvia F. Boj, Hans Clevers, Jeanine M.L. Roodhart, Johannes L. Bos, Hugo J.G. Snippert
Publikováno v:
Cell Reports, Vol 42, Iss 4, Pp 112324- (2023)
Summary: Patient-derived organoids (PDOs) are widely heralded as a drug-screening platform to develop new anti-cancer therapies. Here, we use a drug-repurposing library to screen PDOs of colorectal cancer (CRC) to identify hidden vulnerabilities with
Externí odkaz:
https://doaj.org/article/90d447bcda174d4aba2b71aaf83d4fbd
Autor:
Jia-Yun Chen, Clemens Hug, José Reyes, Chengzhe Tian, Luca Gerosa, Fabian Fröhlich, Bas Ponsioen, Hugo J.G. Snippert, Sabrina L. Spencer, Ashwini Jambhekar, Peter K. Sorger, Galit Lahav
Publikováno v:
Cell Reports, Vol 42, Iss 3, Pp 112252- (2023)
Summary: Oncogene-induced senescence is a phenomenon in which aberrant oncogene expression causes non-transformed cells to enter a non-proliferative state. Cells undergoing oncogenic induction display phenotypic heterogeneity, with some cells senesci
Externí odkaz:
https://doaj.org/article/36dd9f1bb081478bb61988811e5931cb
Autor:
Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure intra
Externí odkaz:
https://doaj.org/article/28e02e090b82435fa3bc07ed281bc827
Autor:
Yannik Bollen, Joris H Hageman, Petra van Leenen, Lucca L M Derks, Bas Ponsioen, Julian R Buissant des Amorie, Ingrid Verlaan-Klink, Myrna van den Bos, Leon W M M Terstappen, Ruben van Boxtel, Hugo J G Snippert
Publikováno v:
PLoS Biology, Vol 20, Iss 1, p e3001527 (2022)
CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimu
Externí odkaz:
https://doaj.org/article/cd66eca306e549c4a4b1983de5881ebb
Autor:
Lanette Kempers, Ivo van der Bijl, Anne-Marieke D. van Stalborch, Bas Ponsioen, Coert Margadant
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100690- (2021)
Summary: We describe an optimized, cost-effective, reproducible, and robust protocol to study sprouting angiogenesis in glass-bottom 96-well plates by confocal microscopy, ideal for screening of drug or shRNA libraries. Effective and stable knockdown
Externí odkaz:
https://doaj.org/article/56ea7521564549ae946a0a00a55e8343
Autor:
Alexander Apschner, Leonie F. A. Huitema, Bas Ponsioen, Josi Peterson-Maduro, Stefan Schulte-Merker
Publikováno v:
Disease Models & Mechanisms, Vol 7, Iss 7, Pp 811-822 (2014)
In recent years it has become clear that, mechanistically, biomineralization is a process that has to be actively inhibited as a default state. This inhibition must be released in a rigidly controlled manner in order for mineralization to occur in sk
Externí odkaz:
https://doaj.org/article/f3ed5c23551c4056bd6ff368ac93a62a
Autor:
Carla S Verissimo, René M Overmeer, Bas Ponsioen, Jarno Drost, Sander Mertens, Ingrid Verlaan-Klink, Bastiaan van Gerwen, Marieke van der Ven, Marc van de Wetering, David A Egan, René Bernards, Hans Clevers, Johannes L Bos, Hugo J Snippert
Publikováno v:
eLife, Vol 5 (2016)
Colorectal cancer (CRC) organoids can be derived from almost all CRC patients and therefore capture the genetic diversity of this disease. We assembled a panel of CRC organoids carrying either wild-type or mutant RAS, as well as normal organoids and
Externí odkaz:
https://doaj.org/article/7501d8e3fc1b4a42aa7676d2bb0a2f09
Publikováno v:
PLoS ONE, Vol 3, Iss 4, p e1916 (2008)
We recently reported on CFP-Epac-YFP, an Epac-based single polypeptide FRET reporter to resolve cAMP levels in living cells. In this study, we compared and optimized the fluorescent protein donor/acceptor pairs for use in biosensors such as CFP-Epac-
Externí odkaz:
https://doaj.org/article/19e157788b524ed889098501fb4a0672
Autor:
Sippe G. de Vries, Wigard P. Kloosterman, Holger Rehmann, William Cross, Myrna van den Bos, Sander Boymans, Bingxin Lu, Susanne M.A. Lens, Hugo J. Snippert, Yannik Bollen, Markus J. van Roosmalen, Maximilian Mossner, Nicolle Besselink, Ellen Stelloo, Christopher Kimberley, Edwin Cuppen, Chris P. Barnes, Petra van Leenen, Bas Ponsioen, Ana C. F. Bolhaqueiro, Koen C. Oost, Bastiaan van der Roest, Trevor A. Graham, Andrea Sottoriva, Geert J. P. L. Kops, Leon W.M.M. Terstappen
Publikováno v:
Nature Genetics, 53(8), 1187-1195. Nature Publishing Group
Nature Genetics
Nature genetics, 53(8), 1187-1195. Nature Publishing Group
Nature Genetics
Nature genetics, 53(8), 1187-1195. Nature Publishing Group
Central to tumor evolution is the generation of genetic diversity. However, the extent and patterns by which de novo karyotype alterations emerge and propagate within human tumors are not well understood, especially at single-cell resolution. Here, w
Autor:
Stefan Schulte-Merker, Sasja Blokzijl-Franke, Philippe Herbomel, Jeroen den Hertog, Karima Kissa, Bas Ponsioen, Suma Choorapoikayil
Publikováno v:
Oncogene
Oncogene, 40(15), 2741-2755. Nature Publishing Group
Oncogene, 40(15), 2741-2755. Nature Publishing Group
Hematopoietic stem and progenitor cells (HSPCs) are multipotent cells giving rise to all blood lineages during life. HSPCs emerge from the ventral wall of the dorsal aorta (VDA) during a specific timespan in embryonic development through endothelial