Výsledky vyhledávání - "Barton J. Dear"

Relating Collective Diffusion, Protein–Protein Interactions, and Viscosity of Highly Concentrated Monoclonal Antibodies through Dynamic Light Scattering

Autor: Tony Y. Shay, Carl A. Karouta, Ayush Sharma, Amjad Chowdhury, Jessica J. Hung, Keith P. Johnston, Maria P. Nieto, Kishan Ramachandran, Logan R. Wilks, Barton J. Dear, Thomas M. Truskett, Jason K. Cheung

Publikováno v: Industrial & Engineering Chemistry Research. 58:22456-22471

To understand and predict the viscosities of highly concentrated monoclonal antibody (mAb) solutions, it is important to characterize the protein–protein interactions (PPI) and how they influence t...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d6e2a159f2a08f08392b26692f736a02
https://doi.org/10.1021/acs.iecr.9b03432

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Enhancing Stability and Reducing Viscosity of a Monoclonal Antibody With Cosolutes by Weakening Protein-Protein Interactions

Autor: Logan R. Wilks, Joshua R. Laber, Keith P. Johnston, Jessica J. Hung, Thomas M. Truskett, Barton J. Dear, Ayush Sharma

Publikováno v: Journal of Pharmaceutical Sciences. 108:2517-2526

An understanding of how cosolutes affect the viscosity and storage stability of highly concentrated mAbs as a function of protein-protein interactions (PPIs) would be desirable for improving processing and administration of protein therapeutics. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54a6695735f0c6472d8eda9b5a041a0d
https://doi.org/10.1016/j.xphs.2019.03.008

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Self-diffusion of a highly concentrated monoclonal antibody by fluorescence correlation spectroscopy: insight into protein–protein interactions and self-association

Autor: Jason K. Cheung, Tony Y. Shay, Thomas M. Truskett, Carl C. Hayden, Maria P. Nieto, Barton J. Dear, Carl A. Karouta, Wade F. Zeno, Keith P. Johnston, Amjad Chowdhury, Jeanne C. Stachowiak, Jessica J. Hung, Kishan Ramachandran

Publikováno v: Soft Matter. 15:6660-6676

The dynamic behavior of monoclonal antibodies (mAbs) at high concentration provides insight into protein microstructure and protein-protein interactions (PPI) that influence solution viscosity and protein stability. At high concentration, interpretat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e66f4db2c89ca7b3bdce0c4f43f8e98e
https://doi.org/10.1039/c9sm01071h

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Coarse-Grained Molecular Dynamics Simulations for Understanding the Impact of Short-Range Anisotropic Attractions on Structure and Viscosity of Concentrated Monoclonal Antibody Solutions

Autor: Jason K. Cheung, Keith P. Johnston, Amjad Chowdhury, Barton J. Dear, Thomas M. Truskett, Jonathan A. Bollinger

Publikováno v: Molecular pharmaceutics. 17(5)

Understanding protein-protein interactions in concentrated therapeutic monoclonal antibody (mAb) solutions is desirable for improved drug discovery, processing, and administration. Here, we deduce both the net protein charge and the magnitude and geo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2edb670d6a0b8833d544ba0897bb7f9
https://pubmed.ncbi.nlm.nih.gov/32101441

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Charge Shielding Prevents Aggregation of Supercharged GFP Variants at High Protein Concentration

Autor: Thomas M. Truskett, Jennifer A. Maynard, Matheus L. Martins, Joshua R. Laber, Jimmy Gollihar, Devin E. Jackson, Barton J. Dear, Andrea M. DiVenere, Keith P. Johnston, Andrew D. Ellington

Publikováno v: Molecular Pharmaceutics. 14:3269-3280

Understanding protein stability is central to combatting protein aggregation diseases and developing new protein therapeutics. At the high concentrations often present in biological systems, purified proteins can exhibit undesirable high solution vis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bff270f3593fb783fd047ac758b2705
https://doi.org/10.1021/acs.molpharmaceut.7b00322

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X-ray Scattering and Coarse-Grained Simulations for Clustering and Interactions of Monoclonal Antibodies at High Concentrations

Autor: P. Douglas Godfrin, Jessica J. Hung, Tony Y. Shay, Jason K. Cheung, Ayush Sharma, Keith P. Johnston, Maria P. Nieto, Amjad Chowdhury, Thomas M. Truskett, Dmytro Nykypanchuk, Jonathan A. Bollinger, Barton J. Dear, Carl A. Karouta, Kishan Ramachandran, Logan R. Wilks

Publikováno v: The journal of physical chemistry. B. 123(25)

Attractive protein?protein interactions (PPI) in concentrated monoclonal antibody (mAb) solutions may lead to reversible oligomers (clusters) that impact colloidal stability and viscosity. Herein, the PPI are tuned for two mAbs via the addition of ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb07e838aca0990d52e38a8794c881ed
https://pubmed.ncbi.nlm.nih.gov/31146525

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Protein-Protein Interactions of Highly Concentrated Monoclonal Antibody Solutions via Static Light Scattering and Influence on the Viscosity

Autor: Jonathan A. Bollinger, Jason K. Cheung, P. Douglas Godfrin, Amjad Chowdhury, Barton J. Dear, Thomas M. Truskett, Ayush Sharma, Jessica J. Hung, Kishan Ramachandran, Keith P. Johnston, Tony Y. Shay, Carl A. Karouta, Maria P. Nieto, Logan R. Wilks

Publikováno v: The journal of physical chemistry. B. 123(4)

The ability to design and formulate mAbs to minimize attractive interactions at high concentrations is important for protein processing, stability, and administration, particularly in subcutaneous delivery, where high viscosities are often challengin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2ae31138bd751a62e26da14ac86505d
https://pubmed.ncbi.nlm.nih.gov/30614707

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Viscosity Reduction of a Concentrated Monoclonal Antibody with Arginine·HCl and Arginine·Glutamate

Autor: Jingyan Yue, Barton J. Dear, Aileen K. Dinin, Jessica J. Hung, Brian K. Wilson, April Twu, Ameya Borwankar, Thomas M. Truskett, Jennifer A. Maynard, Keith P. Johnston

Publikováno v: Industrial & Engineering Chemistry Research. 55:11225-11234

To further advance a subcutaneous injection of monoclonal antibodies (mAbs) at elevated concentrations, novel concepts are needed to lower the viscosity. The addition of high concentrations of cosolutes, namely, arginine glutamate (Arg·Glu) or Arg·

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2efd322ae7ed6de0121529a2263ba619
https://doi.org/10.1021/acs.iecr.6b02042

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High concentration tangential flow ultrafiltration of stable monoclonal antibody solutions with low viscosities

Autor: Jessica J. Hung, Keith P. Johnston, Barton J. Dear, Ameya Borwankar, Thomas M. Truskett

Publikováno v: Journal of Membrane Science. 508:113-126

During production of concentrated monoclonal antibody formulations by tangential flow ultrafiltration (TFF), high viscosities and aggregation often cause extensive membrane fouling, flux decay and low product yields. To address these challenges, the

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Improving Viscosity and Stability of a Highly Concentrated Monoclonal Antibody Solution with Concentrated Proline

Autor: Ameya Borwankar, Thomas T Truskett, Barton J. Dear, Jessica J. Hung, Aileen K. Dinin, Sumarth K. Mehta, Keith P. Johnston

Publikováno v: Pharmaceutical research. 35(7)

To explain the effects of the osmolyte proline on the protein-protein interactions (PPI), viscosity and stability of highly concentrated antibody solutions in contrast to other neutral osmolytes. The viscosity of ~225 mg/mL mAb solutions was measured

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba76e937b5e6a82f77e68f2e0a18d830
https://pubmed.ncbi.nlm.nih.gov/29713822

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