Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Barry R. Ganong"'
Autor:
Barry R. Ganong, Shi-Mei Wang
Publikováno v:
Biochemical and Biophysical Research Communications. 186:562-568
Summary Solubilized phosphatidylinositol-4-phosphate 4-phosphatase from bovine brain resolved into two peaks of activity by ion exchange chromatography. Both exhibited substantial detergent binding characteristic of integral membrane proteins, and bo
Autor:
John P. Delmore, Barry R. Ganong
Publikováno v:
Analytical Biochemistry. 193:35-37
Triton X-114 solutions separate above 22 degrees C into two immiscible aqueous phases. The more dense phase is enriched in detergent, and the less dense phase is depleted of detergent, relative to the original single phase. This phenomenon has been u
Autor:
Barry R. Ganong
Publikováno v:
Biochemistry. 29(29)
Phosphatidylinositol (PI) kinase activity was solubilized from rat liver microsomes and partially purified by chromatography on hydroxyapatite and Reactive Green 19-Superose. Examination of the ATP dependence using a mixed micellar assay gave a Km of
Autor:
Barry R. Ganong
Publikováno v:
Journal of Chemical Education. 77:1606
A portable conductivity meter and probe are described. They have a unique combination of features that permits use with small volumes in the laboratory or in the field. The probe consists of two graphite electrodes embedded in a rigid plastic tube wi
Publikováno v:
Journal of Biological Chemistry. 261:6993-7000
Diacylglycerol kinase is though to play a central role in the metabolism of diacylglycerol second messengers in agonist-stimulated cells. A series of diacylglycerol analogs were tested for their ability to act as substrates or inhibitors of diacylgly
Publikováno v:
Journal of Biological Chemistry. 260:1358-1361
The ability of exogenous sn-1,2-diacylglycerols and analogs to function as bioregulators of protein kinase C in human platelets was investigated. The activation of protein kinase C in platelets is indicated by specific phosphorylation of a 40,000-dal
Autor:
James Ebeling, Barry R. Ganong, Robert M. Bell, James E. Niedel, G. R. Vandenbark, Linda J. Kuhn
Publikováno v:
Proceedings of the National Academy of Sciences. 82:815-819
Activation of cellular protein kinase C appears to be involved in the mechanism by which phorbol diesters induce differentiation of human myeloid leukemia cells (HL-60). Protein kinase C is thought to be physiologically activated by diacylglycerol de
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 796:373-383
A new method for studying phospholipid biosynthesis in Escherichia coli is described. The method makes use of the previously reported observation that E. coli cytidine auxotrophs accumulate phosphatidic acid when starved of cytidine (Ganong, B. and R
Publikováno v:
Proceedings of the National Academy of Sciences. 83:1184-1188
The specificity of protein kinase C activation by sn-1,2-diacylglycerols and analogues was investigated by using a Triton X-100 mixed micellar assay [Hannun, Y. A., Loomis, C. R. & Bell, R. M. (1985) J. Biol. Chem. 260, 10039-10043]. Analogues contai
Publikováno v:
Biochemical and Biophysical Research Communications. 121:386-391
1,2-Diacylglycerol has recently been reported to potentiate the ability of phospholipases A and C to hydrolyze phospholipids in a cellfree system. The present study has been undertaken to investigate whether 1,2-diacylglycerol can also perform this f