Zobrazeno 1 - 10
of 67
pro vyhledávání: '"Barry Polisky"'
Publikováno v:
BioTechniques, Vol 32, Iss 4, Pp 934-938 (2002)
We have developed a rapid and sensitive thin film assay for in-process monitoring of target protein purification. This novel biosensor method provides rapid (5-min) visual evaluation of column purification fractions. The method can be used to monitor
Externí odkaz:
https://doaj.org/article/7d3cec31da4d4fb8a08877b9d4d3f7dd
Autor:
Yan Chen, Erin Kwang, Rachel E. Johns, Renata Fam, Michael McCutcheon, Feng Chen, Alan So, Gregory Severson, Barry Polisky, Michael V. Templin, Yoshiyuki Matsui, Roger C. Adami, Tod Brown, Kathy Fosnaugh, Iwona M. Maciagiewicz, Pierrot Harvie, Yan Liu, Shaguna Seth, Akihide Takagi, Narendra K. Vaish, Tianying Zhu, Susan Bell, Ken Farber, Michael E. Houston, Patrick Charmley
Publikováno v:
Molecular Therapy. 19(5):928-935
Harnessing RNA interference (RNAi) to silence aberrant gene expression is an emerging approach in cancer therapy. Selective inhibition of an overexpressed gene via RNAi requires a highly efficacious, target-specific short interfering RNA (siRNA) and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62796ee00e373a530a6d2ac07ea65b9e
Autor:
Michael E. Houston, Brian R. Granger, Yan Liu, Gregory Severson, Shaguna Seth, Barry Polisky, Roger C. Adami, Rachel E. Johns, Feng Chen, Patrick Charmley, Pierrot Harvie, Tod Brown, Kathy Fosnaugh, Narendra K. Vaish, Michael V. Templin, Yan Chen
Publikováno v:
Nucleic Acids Research
siRNAs confer sequence specific and robust silencing of mRNA. By virtue of these properties, siRNAs have become therapeutic candidates for disease intervention. However, their use as therapeutic agents can be hampered by unintended off-target effects
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41928dbf8a5d14e870ebb8ce4940d271
http://europepmc.org/articles/PMC3061082
http://europepmc.org/articles/PMC3061082
Autor:
Karl Kossen, Toshiyuki Koya, Erwin W. Gelfand, Barry Polisky, Joseph J. Lucas, Shawn Zinnen, Tricia N. Lively, Ivan Richards, Annette Balhorn, Katsuyuki Takeda
Publikováno v:
The Journal of Allergy and Clinical Immunology
Background RNA interference is an endogenous cellular mechanism in which short interfering RNAs (siRNAs) direct the sequence specific degradation of a target mRNA. siRNAs can be synthesized with chemical modifications to increase stability and reduce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::977240a92dac71c8041fe1b55f14258f
https://doi.org/10.1016/j.jaci.2007.08.029
https://doi.org/10.1016/j.jaci.2007.08.029
Autor:
Narendra K. Vaish, Christopher Pasko, Karl Kossen, Vasant Jadhav, Hong Wang, Barry Polisky, Robert D. Jenison, Scott D Seiwert, James McSwiggen
Publikováno v:
Chemistry & Biology. 11:807-815
Many reports have suggested that target-activated ribozymes hold potential value as detection reagents. We show that a "half"-ribozyme ligase is activated similarly by three unstructured oligoribonucleotides representing the major sequence variants o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d4151b1f9b782cbadec960090300cb5
https://doi.org/10.1016/j.chembiol.2004.03.029
https://doi.org/10.1016/j.chembiol.2004.03.029
Autor:
James McSwiggen, Scott D. Seiwert, Barry Polisky, Narendra K. Vaish, Christopher Pasko, Vasant Jadhav, Karl Kossen, Lori E. Andrews
Publikováno v:
RNA. 9:1058-1072
We describe a strategy for the ultra-sensitive detection of nucleic acids using “half” ribozymes that are devoid of catalytic activity unless completed by a trans-acting target nucleic acid. The half-ribozyme concept was initially demonstrated us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ec285eb988244f201b4eda41cb2e1b1
https://doi.org/10.1261/rna.5760703
https://doi.org/10.1261/rna.5760703
Publikováno v:
Biosensors and Bioelectronics. 16:757-763
Rapid, sensitive assays for nucleic acid amplification products have utility for the identification of bacterial or viral infections. We have developed a nucleic acid hybridization assay utilizing thin film technology that permits visual detection of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9017ca247683bc462df3ac810abfe6c3
https://doi.org/10.1016/s0956-5663(01)00217-2
https://doi.org/10.1016/s0956-5663(01)00217-2
Publikováno v:
Clinical Chemistry. 47:1894-1900
Background: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format.Methods: Capture probes were immobilized either passively
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eaceeca381d2a169f77c99dcafeb58c1
https://doi.org/10.1093/clinchem/47.10.1894
https://doi.org/10.1093/clinchem/47.10.1894
Autor:
James W. Meador, Helen La, Anna Ettinger, Rachel Ostroff, Barry Polisky, Leora S. Zalman, Amy K. Patick, Steve Worland, Marynette Rihanek
Publikováno v:
Journal of Clinical Virology
Background: More than 100 immunologically distinct serotypes of human rhinoviruses (HRV) have been discovered, making detection of surface exposed capsid antigens impractical. However, the non-structural protein 3C protease (3Cpro) is essential for v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4aa64dd9782ebcac09fe80ee2bfe964
http://europepmc.org/articles/PMC7128216
http://europepmc.org/articles/PMC7128216
Publikováno v:
Clinical Chemistry. 46:1501-1504
The reported incidence of methicillin-resistant Staphylococcus aureus (MRSA) isolates in hospitals has increased from 2% in 1974 to 50% in 1997 (1). In addition, MRSA is emerging as a community-acquired pathogen. Resistance is most often mediated by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::84d0ee7d9a93817db68cfa3c4e6f0003
https://doi.org/10.1093/clinchem/46.9.1501
https://doi.org/10.1093/clinchem/46.9.1501