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pro vyhledávání: '"Barry J. Bowman"'
Autor:
Barry J. Bowman
Publikováno v:
PLoS ONE, Vol 18, Iss 4 (2023)
The hyphal tips of Neurospora crassa have prevacuolar compartments (PVCs) of unusual size and shape. They appear to function as late endosomes/multivesicular bodies. PVCs are highly variable in size (1–3 microns) and exhibit rapid changes in struct
Externí odkaz:
https://doaj.org/article/8c6f504102b547ae9a57e68ee9083794
Publikováno v:
Frontiers in Microbiology
Frontiers in Microbiology, Vol 10 (2019)
Frontiers in Microbiology, Vol 10 (2019)
The NADPH oxidases (NOX) catalyze the production of superoxide by transferring electrons from NADPH to O2, in a regulated manner. In Neurospora crassa NOX-1 is required for normal growth of hyphae, development of aerial mycelium and asexual spores, a
Autor:
Barry J. Bowman, Peter Novick, Constanze Seidel, Reinhard Fischer, Meritxell Riquelme, Eddy Sánchez-León
Publikováno v:
Molecular Microbiology. 95:472-490
Vesicle traffic involves budding, transport, tethering and fusion of vesicles with acceptor membranes. GTP-bound small Rab GTPases interact with the membrane of vesicles, promoting their association with other factors before their subsequent fusion.
Publikováno v:
Eukaryotic Cell. 12:1097-1105
Most models for fungal growth have proposed a directional traffic of secretory vesicles to the hyphal apex, where they temporarily aggregate at the Spitzenkörper before they fuse with the plasma membrane (PM). The PM H + -translocating ATPase (PMA-1
Autor:
Ernestina Castro-Longoria, Stephen J. Free, Michael K. Watters, Stephan Seiler, Barry J. Bowman, Meritxell Riquelme, Corinna Richthammer, André Fleißner, Oded Yarden, Salomon Bartnicki-Garcia, Eddy Sánchez-León, Robert W. Roberson, Carolyn G. Rasmussen, Michael Freitag, Rosa R. Mouriño-Pérez, Michael Plamann, Roger R. Lew
Publikováno v:
Fungal Biology. 115:446-474
Neurospora crassa has been at the forefront of biological research from the early days of biochemical genetics to current progress being made in understanding gene and genetic network function. Here, we discuss recent developments in analysis of the
Publikováno v:
Eukaryotic Cell. 10:654-661
We have examined the distribution of calcium inNeurospora crassaand investigated the role of four predicted calcium transport proteins. The results of cell fractionation experiments showed 4% of cellular calcium in mitochondria, approximately 11% in
Publikováno v:
Eukaryotic Cell. 8:1845-1855
We wanted to examine the cellular locations of four Neurospora crassa proteins that transport calcium. However, the structure and distribution of organelles in live hyphae of N. crassa have not been comprehensively described. Therefore, we made recom
Autor:
Barry J. Bowman, Emma Jean Bowman, Robert R. Schnittker, Marija Draskovic, Meritxell Riquelme, Michael Plamann, Tarik El-Mellouki, Eddy Sánchez-León
Publikováno v:
Eukaryotic cell, vol 14, iss 12
Using confocal microscopy, we observed ring-like organelles, similar in size to nuclei, in the hyphal tip of the filamentous fungus Neurospora crassa . These organelles contained a subset of vacuolar proteins. We hypothesize that they are novel preva
Publikováno v:
Journal of Biological Chemistry. 281:31885-31893
The vacuolar ATPase has been implicated in a variety of physiological processes in eukaryotic cells. Bafilomycin and concanamycin, highly potent and specific inhibitors of the vacuolar ATPase, have been widely used to investigate the enzyme. Derivati
Publikováno v:
Journal of Biological Chemistry. 281:27052-27062
To address questions about the structure of the vacuolar ATPase, we have generated mutant strains of Neurospora crassa defective in six subunits, C, H, a, c, c′, and c″. Except for strains lacking subunit c′, the mutant strains were indistingui