Zobrazeno 1 - 10
of 365
pro vyhledávání: '"Barry L. Karger"'
Autor:
Ioanna Tzani, Marina Castro-Rivadeneyra, Paul Kelly, Lisa Strasser, Lin Zhang, Martin Clynes, Barry L. Karger, Niall Barron, Jonathan Bones, Colin Clarke
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract Chinese hamster ovary (CHO) cells are used to produce almost 90% of therapeutic monoclonal antibodies (mAbs) and antibody fusion proteins (Fc-fusion). The annotation of non-canonical translation events in these cellular factories remains inc
Externí odkaz:
https://doaj.org/article/5f963831bb07487b811c1a7ac3b1468f
Autor:
Lisa Strasser, Amy Farrell, Jenny T. C. Ho, Kai Scheffler, Ken Cook, Patrick Pankert, Peter Mowlds, Rosa Viner, Barry L. Karger, Jonathan Bones
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in combination with optimization of various process parameters results in incr
Externí odkaz:
https://doaj.org/article/ee7e97d3845d46d8bf5f4e669556f480
Publikováno v:
Analytical Chemistry. 93:9267-9276
Recent progress in the development and production of new, innovative protein therapeutics require rapid and adjustable high-resolution bioseparation techniques. Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) using a borate (B) cross-l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d75b1aaf8e6eba650fd6e2ae215dde4c
https://doi.org/10.1021/acs.analchem.1c01636
https://doi.org/10.1021/acs.analchem.1c01636
Autor:
Xiaomin Song, Bin Li, Yan Xiao, Chunxia Chen, Qiang Wang, Yujie Liu, Alan Berezov, Chen Xu, Yayi Gao, Zhiyuan Li, Shiaw-Lin Wu, Zheng Cai, Hongtao Zhang, Barry L. Karger, Wayne W. Hancock, Andrew D. Wells, Zhaocai Zhou, Mark I. Greene
Publikováno v:
Cell Reports, Vol 1, Iss 6, Pp 665-675 (2012)
FOXP3 is a key transcription factor for regulatory T cell function. We report the crystal structure of the FOXP3 coiled-coil domain, through which a loose or transient dimeric association is formed and modulated, accounting for the activity variation
Externí odkaz:
https://doaj.org/article/fcde7d29afb943fca110e332fa7543d3
Autor:
Marina Castro-Rivadeneyra, Ioanna Tzani, Paul Kelly, Lisa Strasser, Felipe Guapo, Ciara Tierney, Michelle Chain, Lin Zhang, Martin Clynes, Barry L. Karger, Niall Barron, Jonathan Bones, Colin Clarke
Chinese hamster ovary (CHO) cells are used to produce almost 90% of therapeutic monoclonal antibodies (mAbs). The annotation of non-canonical translation events in these cellular factories remains incomplete, limiting not only our ability to study CH
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4e542ec0a048420331458321dd0f2797
https://doi.org/10.1101/2022.01.20.475618
https://doi.org/10.1101/2022.01.20.475618
Autor:
Kai Scheffler, Ken Cook, Peter Mowlds, Patrick Pankert, Jenny Ho, Amy Farrell, Rosa Viner, Jonathan Bones, Barry L. Karger, Lisa Strasser
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology
The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in combination with optimization of various process parameters results in incr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b15ae8b530928c89f749b4b26dbbec9d
https://www.frontiersin.org/articles/10.3389/fbioe.2021.569045/full
https://www.frontiersin.org/articles/10.3389/fbioe.2021.569045/full
Autor:
Roberto Sebastiano, Alexander R. Ivanov, Marcia R. Santos, Li Zang, Arseniy M. Belov, Barry L. Karger, David R. Bush
Publikováno v:
ELECTROPHORESIS. 39:2069-2082
High-resolution capillary zone electrophoresis - mass spectrometry (CZE-MS) has been of increasing interest for the analysis of biopharmaceuticals. In this work, a combination of middle-down and intact CZE-MS analyses has been implemented for the cha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::561e4804bfa29df4862adaf6ede9359c
https://doi.org/10.1002/elps.201800067
https://doi.org/10.1002/elps.201800067
Autor:
Nesredin Mussa, Simion Kreimer, Mi Jin, Somak Ray, Yuanwei Gao, Alexander R. Ivanov, Zheng Jian Li, Li Tao, Barry L. Karger, Zhijun Tan
Publikováno v:
Analytical Chemistry. 89:5294-5302
Host cell proteins (HCPs) are process-related impurities of biopharmaceuticals that remain at trace levels despite multiple stages of downstream purification. Currently, there is interest in implementing LC-MS in biopharmaceutical HCP profiling along
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c5ec1dbe13e6d32726bdeba7103b7be
https://doi.org/10.1021/acs.analchem.6b04892
https://doi.org/10.1021/acs.analchem.6b04892
Autor:
Rachel Chen, Ravali Raju, Rashmi Kshirsagar, Amr S. Ali, Barry L. Karger, Li Zang, Alan Gilbert, Alexander R. Ivanov
Publikováno v:
Biotechnol J
Chinese hamster ovary (CHO) cells are currently the primary host cell lines used in biotherapeutic manufacturing of monoclonal antibodies (mAbs) and other biopharmaceuticals. Cellular energy metabolism and endoplasmic reticulum (ER) stress are known
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbe89edb5516d763dc53d6c19f1b7a82
https://pubmed.ncbi.nlm.nih.gov/32170810
https://pubmed.ncbi.nlm.nih.gov/32170810
Autor:
Arseniy M. Belov, Li Zang, Roberto Sebastiano, Marcia R. Santos, David R. Bush, Barry L. Karger, Alexander R. Ivanov
Publikováno v:
ELECTROPHORESIS. 39
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ff578af273a191f693994ea255303b2
https://doi.org/10.1002/elps.201870132
https://doi.org/10.1002/elps.201870132