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3C in maize and arabidopsis

Autor: Weber, B., Jamge, S., Stam, M., Bemer, M., Baroux, C.

Publikováno v: Plant Chromatin Dynamics: Methods and Protocols, 247-270
STARTPAGE=247;ENDPAGE=270;TITLE=Plant Chromatin Dynamics
Plant Chromatin Dynamics
Methods in Molecular Biology ISBN: 9781493973170
Plant Chromatin Dynamics. Humana Press Inc.

With Chromosome Conformation Capture (3C), the relative interaction frequency of one chromosomal fragment with another can be determined. The technique is especially suited for unraveling the 3D organization of specific loci when focusing on aspects

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bbc30535ec76d4ecf1428e69fb5ef8c
https://doi.org/10.1007/978-1-4939-7318-7_15

Fluorescence In Situ Hybridization (FISH) and Immunolabeling on 3D Preserved Nuclei

Autor: Bey, T.D., Koini, M., Fransz, P., Bemer, M., Baroux, C.

Publikováno v: Methods in Molecular Biology ISBN: 9781493973170
Plant Chromatin Dynamics: Methods and Protocols, 467-480
STARTPAGE=467;ENDPAGE=480;TITLE=Plant Chromatin Dynamics

The spatial distribution of genes in the nucleus emerges as an important factor in gene regulation and epigenetics. The position of loci relative to each other, to nuclear landmarks such as the nucleolus and chromocenters, as well as to chromatin pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b90dc31d08ff130b1d2846b751a1487c
https://doi.org/10.1007/978-1-4939-7318-7_27

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Efficient and rapid isolation of early-stage embryos from Arabidopsis thaliana seeds

Autor: Raissig, M. T., Gagliardini, V., Jaenisch, J., Ueli Grossniklaus, Baroux, C.

Publikováno v: Journal of visualized experiments : JoVE
Scopus-Elsevier

In flowering plants, the embryo develops within a nourishing tissue - the endosperm - surrounded by the maternal seed integuments (or seed coat). As a consequence, the isolation of plant embryos at early stages (1 cell to globular stage) is technical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::a6faebd5c30d8ccf6aeb54670da99c3c
https://doi.org/10.5167/uzh-89709