Zobrazeno 1 - 10
of 79
pro vyhledávání: '"Barend Kraal"'
Publikováno v:
European Journal of Biochemistry. 227:816-822
Pulvomycin is a strong inhibitor of protein synthesis, known to prevent the binding of aminoacyl-tRNA to elongation factor Tu · GTP (EF-Tu · GTP). Recently, three pulvomycin-resistant mutant strains have been isolated by targeted mutagenesis of the
Autor:
Barend Kraal, F. J. Duisterwinkel, Peter J. M. Schretlen, Leendert Bosch, J. Martiea De Graaf
Publikováno v:
European Journal of Biochemistry. 117:7-12
In the accompanying paper we have shown that polypeptide synthesis sustained by the mutant elongation factor EF-TuBO is inhibited by kirromycin. Here we have searched for the primary site of inhibition in the elongation cycle. It is demonstrated that
Autor:
Brian F.C. Clark, Sander G.J. Mathu, Charlotte R. Knudsen, Barend Kraal, Henrik Karring, Jan van Duin
Publikováno v:
Journal of Biological Chemistry. 279:1878-1884
Elongation factor Ts (EF-Ts) is the guanine-nucleotide exchange factor of elongation factor Tu (EF-Tu), which promotes the binding of aminoacyl-tRNA to the mRNA-programmed ribosome in prokaryotes. The EF-Tu.EF-Ts complex, one of the EF-Tu complexes d
Publikováno v:
Journal of Chromatography B. 786:279-286
The RNA genome of coliphage Qbeta is replicated by a complex of four proteins, one of them being the translation elongation factor Tu. The role of EF-Tu in this RNA polymerase complex is still unclear, but the obligate presence of translationally fun
Autor:
Hugo H. J. Bink, Cornelis W. A. Pleij, Barend Kraal, Sharief Barends, Sjoerd H. E. van den Worm
Publikováno v:
Cell. 112:123-129
Turnip yellow mosaic virus (TYMV) has a genomic plus-strand RNA with a 5' cap followed by overlapping and different reading frames for the movement protein and polyprotein, while the distal coat protein cistron is translated from a subgenomic RNA. Th
Publikováno v:
European Journal of Biochemistry. 268:3807-3815
In Streptomyces coelicolor, the regular and abundant elongation factor (EF)-Tu1 is encoded by tuf1, while the actual function of the highly divergent tuf3 gene product is not yet known. Expression of the latter could so far only be detected on the tr
Publikováno v:
Biochemistry. 39:2652-2658
Aminoacylation and transportation of tmRNA to stalled ribosomes constitute prerequisite steps for trans-translation, a process facilitating the release of stalled ribosomes from 3' ends of truncated mRNAs and the degradation of incompletely synthesiz
Publikováno v:
Antonie van Leeuwenhoek. 78:269-276
This paper describes the effects of increased expression of the cell division genes ftsZ, ftsQ, and ssgA on the development of both solid- and liquid-grown mycelium of Streptomyces coelicolor and Streptomyces lividans. Over-expression of ftsZ in S. c
Publikováno v:
Journal of Biochemical and Biophysical Methods. 42:1-14
Elongation factor Tu is essential for binding and a correct delivery of aminoacyl-tRNA during protein biosynthesis. For a good characterization of its interaction with tRNA in terms of structure-function relationship, determinations of kinetic equili
Autor:
J.Martien de Graaf, Anne-Marie Zuurmond, Barend Kraal, Lian N. Olsthoorn-Tieleman, Andrea Parmeggiani
Publikováno v:
Journal of Molecular Biology. 294:627-637
For clarification of the action of a new antibiotic, the analysis of resistant mutants is often indispensable. For enacyloxin IIa we discovered four resistant elongation factor Tu (EF-Tu) species in Escherichia coli with the mutations Q124K, G316D, Q