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pro vyhledávání: '"Barbara Pedrotti"'
Autor:
Khalid Islam, Barbara Pedrotti
Publikováno v:
FEBS Letters. 403:123-126
The effect of estramustine and estramustine phosphate (EP) on the interaction of microtubule associated protein 2 (MAP2) with actin has been examined. We show that (a) neither estramustine nor EP influences actin polymerisation (b) EP, but not estram
Publikováno v:
Cell Motility and the Cytoskeleton. 35:134-146
The regulation of microtubule dynamics in vitro by microtubule-associated proteins (MAPs) was examined, using purified porcine MAP1B and MAP2. MAP1B has a significantly smaller effect on the observed critical concentration for microtubule assembly th
Autor:
Barbara Pedrotti, Khalid Islam
Publikováno v:
Cell Motility and the Cytoskeleton. 30:301-309
A simple procedure for the purification of MAP1B from bovine brain is described. The procedure requires two ion-exchange chromatographic steps and results in > 95% pure MAP1B with a typical recovery of about 25-30 mg/kg of brain tissue. SDS-PAGE anal
Autor:
Khalid Islam, Barbara Pedrotti
Publikováno v:
Biochemistry. 33:12463-12470
In a recent study, we have shown that sulfonate buffers affect microtubule assembly and alter microtubule protein composition (Pedrotti et al., 1993). In particular, we noted that PIPES buffer leads to removal of MAP1 from the microtubule surface wit
Publikováno v:
Biochemistry. 33:8798-8806
A solid-phase immunoassay is used to study the protein-protein interactions between microtubule-associated protein MAP2 and the cytoskeletal proteins tubulin and actin. The assay can be performed on 96-well microtiter plates and can be used to study
Publikováno v:
Cell Motility and the Cytoskeleton. 29:110-116
High molecular weight microtubule-associated proteins MAP1A and MAP2 form thin projections from microtubule surfaces and have been implicated in crosslinking microtubules and other cytoskeletal components. We have purified native MAP1A from bovine br
Publikováno v:
Cell Motility and the Cytoskeleton. 25:234-242
The influence of two commonly used sulphonate buffers, PIPES and MES, on the in vitro assembly of bovine brain microtubule protein was examined. Microtubule assembly was monitored by turbimetry and, after centrifugation, the polymerised protein was a
Dephosphorylated but not phosphorylated microtubule associated protein MAP1B binds to microfilaments
Autor:
Barbara Pedrotti, Khalid Islam
Publikováno v:
FEBS Letters. 388:131-133
We have reported that purified native MAP1B interacts with microtubules but not with microfilaments [Pedrotti and Islam, Cell Motil. Cytoskel. (1995) 30, 301–309]. However, MAP1B can be phosphorylated at multiple sites by casein kinase II (CKII) an
Publikováno v:
Cell biology international. 25(10)
The microtubular system is emerging as a cell target in neurodegeneration evoked by the Parkinsonism-inducing neurotoxin N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its toxic metabolite N-methyl-4-phenylpyridinium (MPP(+)). Looking for a
Autor:
Barbara Pedrotti
Publikováno v:
Journal of Orthopaedics and Traumatology : Official Journal of the Italian Society of Orthopaedics and Traumatology