Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Barbara Kure Klein"'
Publikováno v:
Biochemistry. 34:6540-6551
Interleukin-3 (IL-3) is a cytokine which stimulates the proliferation and differentiation of hematopoietic progenitors into multiple cell lineages. The 1H, 15N, and 13C NMR resonances of a recombinant human IL-3 variant (SC-65369) have been assigned
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55e51d9433e0d1c0d4b574ff4b75ef75
https://doi.org/10.1021/bi00019a036
https://doi.org/10.1021/bi00019a036
Publikováno v:
Gene. 101:291-295
Bovine insulin-like growth factor 2 (bIGF2) was produced in inclusion bodies in the cytoplasm of Escherichia coli and accumulated at high levels: 20-25% of total Coomassie-stained bacterial protein. The level of accumulation of bIGF2 was affected by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3a5332218f4d91f6416147a3508496f
https://doi.org/10.1016/0378-1119(91)90426-c
https://doi.org/10.1016/0378-1119(91)90426-c
Autor:
Yiqing Feng, and Ann M. Donnelly, Joseph O. Polazzi, Edith S. Grabbe, Jeng-Jong Shieh, Barbara Kure Klein, Kumnan Paik, Linda L. Zurfluh, Mark P. Baganoff, John P. McKearn, Charles A. McWherter, William F. Hood, Ann L. Abegg
Publikováno v:
Biochemistry. 38(14)
Myelopoietins (MPOs) are a family of engineered dual interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF) receptor agonists that are superior in comparison to the single agonists in their ability to promote the growth and maturation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::922d5df240c06e04c290d67fa2c4ff50
https://pubmed.ncbi.nlm.nih.gov/10194378
https://pubmed.ncbi.nlm.nih.gov/10194378
Autor:
Kumnan Paik, Kris Sterbenz, Barrett R. Thiele, Sarah Ruth Braford-Goldberg, John P. McKearn, Barbara Kure Klein, S. Christopher Bauer, Alan Michael Easton, Peter O. Olins, Joseph O. Polazzi, Jon A. Klover, Maire Helena Caparon
Publikováno v:
The Journal of biological chemistry. 270(40)
A deletion variant of human interleukin-3, hIL-3(15-125), was produced in the periplasmic space of Escherichia coli and had full activity in an AML193.1.3 cell proliferation assay. Libraries of random single-amino acid substitutions were constructed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cad04817236bc23faf3e9c2e7c961cb
https://pubmed.ncbi.nlm.nih.gov/7559548
https://pubmed.ncbi.nlm.nih.gov/7559548
Autor:
N.R. Staten, Barbara Kure Klein, John Warren Thomas, John P. McKearn, Mark Allen Abrams, Kumnan Paik, Charles M. Baum, William F. Hood, Joseph B. Monahan
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 92(9)
A systematic evaluation of structure-activity information led to the construction of genetically engineered interleukin 3 (IL-3) receptor agonists (synthokines) with enhanced hematopoietic potency. SC-55494, the most extensively characterized member
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f235d31e6f8386760644b0e44c672b8
https://pubmed.ncbi.nlm.nih.gov/7537376
https://pubmed.ncbi.nlm.nih.gov/7537376
Autor:
Barbara Kure Klein, Catherine S. Devine, Joseph O. Polazzi, Shaukat H. Rangwala, Peter O. Olins
Publikováno v:
Protein engineering. 5(6)
Bovine somatotropin (bST) was secreted from Escherichia coli at moderate levels of 1-2 micrograms/ml/OD using expression vectors in which the bST gene was fused to the lamB secretion signal. To study the secretion properties of bST in E.coli further,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b42667bb181b61b155085c7a8b88b75
https://pubmed.ncbi.nlm.nih.gov/1438161
https://pubmed.ncbi.nlm.nih.gov/1438161
Autor:
Christine E. Smith, Peter O. Olins, Barbara Kure Klein, Edwin Rowold, Stephen R. Hill, Sybille Galosy, Catherine S. Devine
Publikováno v:
Bio/technology (Nature Publishing Company). 9(9)
We have expressed a chimeric protein, comprising the LamB secretion signal sequence fused to mature bovine somatotropin (bST), in Escherichia coli. Plasmid constructs with the recA promoter showed significant protein accumulation prior to induction a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51e49691ba642fbfb3bfb3a1673d1215
https://pubmed.ncbi.nlm.nih.gov/1367360
https://pubmed.ncbi.nlm.nih.gov/1367360
Autor:
Ganesh M. Kishore, S. C. Bauer, Guy della-Cioppa, Robert T. Fraley, Dilip M. Shah, Barbara Kure Klein
Publikováno v:
Proceedings of the National Academy of Sciences. 83:6873-6877
5- enol Pyruvylshikimate-3-phosphate synthase (EPS P synthase; 3-phosphoshikimate 1-carboxyvinyl-transferase; EC 2.5.1.19) is a chloroplast-localized enzyme of the shikimate pathway in plants. This enzyme is the target for the nonselective herbicide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39ec69cdba66c99e87aef9a2413fc89e
https://doi.org/10.1073/pnas.83.18.6873
https://doi.org/10.1073/pnas.83.18.6873
Publikováno v:
Proceedings of the National Academy of Sciences. 82:3539-3542
Partially denatured 16S rRNA from 30S ribosomes shows features of secondary structure in electron microscopy that correspond to the well accepted secondary structure model derived from chemical modification and phylogenetic data. However, a very diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef51e09175872a89842a413019589809
https://doi.org/10.1073/pnas.82.11.3539
https://doi.org/10.1073/pnas.82.11.3539
Publikováno v:
Biochemistry. 23(17)
Loops observed in partially denatured 16S rRNA lie within three domains, each about 500 nucleotides long. The loops observed in the 5' and central domains agree well with features of the model proposed by Woese et al. [Woese, C. R., Gutell, R., Gupta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd1e369bd340ef8c9fcb8676718997fd
https://pubmed.ncbi.nlm.nih.gov/6207855
https://pubmed.ncbi.nlm.nih.gov/6207855