Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Barbara H. Gibbons"'
Autor:
Ian R. Gibbons, Keiichi Takahashi, Chikako Shingyoji, Barbara H. Gibbons, Kenjiro Yoshimura, Dan Eshel
Publikováno v:
Journal of Experimental Biology. 152:441-451
Within the approximate range of 30–80 Hz, the flagellar beat frequency of a sea urchin sperm held by its head in the tip of a micropipet is governed by the vibration frequency of the micropipet. We have imposed abrupt changes in flagellar beat freq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da60b624d6ea18199020e7de917a1064
https://doi.org/10.1242/jeb.152.1.441
https://doi.org/10.1242/jeb.152.1.441
Transcripts approximately 14.5 kilobases in length from 14 different genes that encode for dynein heavy chains have been identified in poly(A)+ RNA from sea urchin embryos. Analysis of the changes in level of these dynein transcripts in response to d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a249b13957c45fd4fe0c8546ec2e99df
https://europepmc.org/articles/PMC301009/
https://europepmc.org/articles/PMC301009/
Publikováno v:
Biology of the cell. 76(3)
By making the hypothetis that the pattern of conserved sequence residues in the vicinity of the hydrolytic ATP-binding site of dynein would resemble that in myosins from a broad variety of sources, we designed degenerate oligonucleotide primers capab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38a70d3afcdf196d8ea8915765af2d0d
https://pubmed.ncbi.nlm.nih.gov/1305477
https://pubmed.ncbi.nlm.nih.gov/1305477
Autor:
Cheryl A. Phillipson, Wen-Jing Y. Tang, Nathan S. Ching, Gabor Mocz, David J. Asai, I. R. Gibbons, Hening Ren, Gregory J. Dolecki, Barbara H. Gibbons
Publikováno v:
Scopus-Elsevier
A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step, the porti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b9ecb83be477aa3c31b6876e89aa187
https://europepmc.org/articles/PMC52549/
https://europepmc.org/articles/PMC52549/
Publikováno v:
Nature. 352(6336)
AXONEMAL dyneins have two or three globular heads joined by flexible tails to a common base, with each head/tail unit consisting of a single heavy-chain polypeptide of relative molecular mass >400,000. The sizes of the components have been deduced by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55ec6bccc1b1d3454724fe2fc78f64a4
https://pubmed.ncbi.nlm.nih.gov/1830924
https://pubmed.ncbi.nlm.nih.gov/1830924
Autor:
I. R. Gibbons, Barbara H. Gibbons
Publikováno v:
Journal of Muscle Research and Cell Motility. 1:31-59
Flagellar waveforms have been studied during the stopping and starting transients of light-induced, Ca2+-mediated, intermittent swimming of live sperm of the sea urchin Tripneustes gratilla. Tracings of successive frames of movie film made at about 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::899eb4fa941ddea0e28b858475faa039
https://doi.org/10.1007/bf00711924
https://doi.org/10.1007/bf00711924
Autor:
I. R. Gibbons, Barbara H. Gibbons
Publikováno v:
The Journal of Cell Biology
Sea urchin sperm were demembranated and reactivated with a solution containing 0.04% Triton X-100 and 0.03 mM ATP. The ATP concentration was then lowered abruptly by diluting the sperm suspension 50-fold into reactivating solution containing no ATP.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0fd0c18190d77798078e03049f1ed3f
http://europepmc.org/articles/PMC2109359
http://europepmc.org/articles/PMC2109359
Autor:
I. R. Gibbons, Barbara H. Gibbons
Publikováno v:
The Journal of Cell Biology
Sperm flagella of the sea urchin Tripneustes gratilla beat with asymmetrical bending waves after demembranation with Triton X-100 in the presence of EGTA and reactivation at pH 8.1 with 1 mM ATP in the presence of 2 mM MgSO4. Addition of 0.1--0.2 mM
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a935fdb5a4f94c01bef5cfa7f067776c
https://doi.org/10.1083/jcb.84.1.13
https://doi.org/10.1083/jcb.84.1.13
Autor:
Barbara H. Gibbons, I. R. Gibbons
Publikováno v:
Journal of Biological Chemistry. 262:8354-8359
Irradiation of demembranated flagella of sea urchin sperm at 365 nm in the presence of 0.05-1 mM MgATP and 5-10 microM vanadate (Vi) cleaves the alpha and beta heavy chains of the outer arm dynein at the same site and at about the same rate as report
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::60afcb61ac62107feffce13019011504
https://doi.org/10.1016/s0021-9258(18)47571-0
https://doi.org/10.1016/s0021-9258(18)47571-0
Autor:
Gabor Mocz, Barbara H. Gibbons, I. R. Gibbons, A. Lee-Eiford, Wen-Jing Y. Tang, C. A. Phillipson
Publikováno v:
Journal of Biological Chemistry. 262:17728-17734
Irradiation of soluble dynein 1 from sea urchin sperm flagella at 365 nm in the presence of MgATP and 0.05-50 microM vanadate (Vi) cleaves the alpha and beta heavy chains (Mr 428,000) at their V1 sites to give peptides of Mr 228,000 and 200,000, with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2ee5cedb1204ae8135e4a7930804f922
https://doi.org/10.1016/s0021-9258(18)45440-3
https://doi.org/10.1016/s0021-9258(18)45440-3