Výsledky vyhledávání - "Barbara E. H. Coupar"

Purification of Form AI and AII DNA-Dependent RNA Polymerases from Rat-Liver Nucleoli using Low-Ionic-Strength Extraction Conditions

Autor: Barbara E. H. Coupar, C. James Chesterton

Publikováno v: European Journal of Biochemistry. 59:25-34

Recent findings have confirmed the role of form A DNA-dependent polymerase activity as that which is responsible for the transcription of the ribosomal RNA-coding genes. Unfortunately, the form A enzymes have proved to be very labile and difficult to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42cb666c35b6d4b40e2a446a6a812ac0
https://doi.org/10.1111/j.1432-1033.1975.tb02420.x

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The Mechanism by which Heparin Stimulates Transcription in Isolated Rat Liver Nuclei. Polyribonucleotide Elongation Rates and the Number of Transcribing RNA Polymerase Molecules Present

Autor: C. James Chesterton, Barbara E. H. Coupar

Publikováno v: European Journal of Biochemistry. 79:525-533

The polyanion heparin, whilst inhibiting free RNA polymerase, stimulates the rate of RNA synthesis in eucaryotic nuclei; it has been suggested that this process involves either the inactivation of R Nase or the activation of blocked transcription com

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87e547da96288b2d51a9f301ed2708ee
https://doi.org/10.1111/j.1432-1033.1977.tb11837.x

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Transcription of fractionated mammalian chromatin by mammalian ribonucleic acid polymerase. Demonstration of temperature-dependent rifampicin-resistant initiation sites in euchromatin deoxyribonucleic acid

Autor: C. James Chesterton, Peter H. W. Butterworth, Barbara E. H. Coupar

The chromatin fractionation method of Frenster et al. (1963) as modified by Leake et al. (1972) was used to prepare fragments of euchromatin from rat liver nuclei. These remain soluble in 5mm-MgCl2, and contain DNA of maximum mol.wt. 1×106–2×106.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f6db94a0abfca7d73a603b7e7fa664e
https://europepmc.org/articles/PMC1168354/

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Quantification of hepatic transcribing RNA polymerase molecules, polyribonucleotide elongation rates and messenger RNA complexity in fed and fasted rats

Autor: Barbara E. H. Coupar, Jane A. Davies, C. James Chesterton

Publikováno v: European journal of biochemistry. 84(2)

We have investigated the mechanisms by which the rate of preribosomal RNA and premessenger RNA synthesis are repressed during starvation of rats by a quantitative study of the transcriptional events in isolated nuclei and by complexity analysis of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f001622c887666dd8b856256cb1305a4
https://pubmed.ncbi.nlm.nih.gov/639806

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The subunit structure of the form AII DNA-dependent RNA polymerase from rat liver

Autor: Peter H. W. Butterworth, C. James Chesterton, Barbara E. H. Coupar

Publikováno v: FEBS letters. 77(2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12c45a0fb91999fcb91c79018855f63a
https://pubmed.ncbi.nlm.nih.gov/862929

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Studies on the control of ribosomal RNA synthesis in HeLa cells

Autor: Janice Buss, Melvin H. Green, Peter H. W. Butter Worth, C. James Chesterton, Barbara E. H. Coupar

Publikováno v: European journal of biochemistry. 57(1)

In many eucaryotic systems protein synthesis is coupled to ribosomal RNA synthesis such that shut-down of the former causes inhibition of the latter. We have investigated this stringency phenomenon in HeLa cells. The protein synthesis inhibitors cycl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3477c9fdf6b9b5976e91e3b967078957
https://pubmed.ncbi.nlm.nih.gov/1175642

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