Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Barbara, Kaboord"'
Publikováno v:
BioTechniques, Vol 33, Iss 6, Pp 1354-1358 (2002)
The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods,
Externí odkaz:
https://doaj.org/article/36dfd5d0a9894ff79b77c5b1e6689e3a
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2261
Immunoprecipitation (IP) is commonly used upstream of mass spectrometry (MS) as an enrichment tool for low-abundant protein targets. However, several aspects of the classical IP procedure such as nonspecific protein binding to the isolation matrix, d
Publikováno v:
Methods in Molecular Biology ISBN: 9781071611852
Immunoprecipitation (IP) is commonly used upstream of mass spectrometry (MS) as an enrichment tool for low-abundant protein targets. However, several aspects of the classical IP procedure such as nonspecific protein binding to the isolation matrix, d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01261886f2429c45e0673bdc7868a4d0
https://doi.org/10.1007/978-1-0716-1186-9_14
https://doi.org/10.1007/978-1-0716-1186-9_14
Publikováno v:
Cancer Research. 80:2858-2858
Enrichment of cell surface proteins is commonly performed by biotinylation using amine-based chemistry; however, this method is often associated with intracellular labeling and difficulty distinguishing the true cell surface proteins from background
Autor:
Bryan Krastins, Michael Major, Yongjie Ji, Frederick F. Lang, John C. Rogers, Arugadoss Devakumar, Mary F. Lopez, Howard Colman, Carol L. Nilsson, Michael Rosenblatt, Barbara Kaboord, Waldemar Priebe, Gregory Kilmer, Charles A. Conrad, Michael J. Greig, Taha Rezai, Stone D.-H. Shi, David A. Sarracino, Roslyn Dillon, Amol Prakash
Publikováno v:
Journal of Proteome Research. 9:430-443
Initiation and maintenance of several cancers including glioblastoma (GBM) may be driven by a small subset of cells called cancer stem cells (CSCs). CSCs may provide a repository of cells in tumor cell populations that are refractory to chemotherapeu
Publikováno v:
The FASEB Journal. 29
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1295
Immunoprecipitation (IP) is commonly used upstream of mass spectrometry (MS) as an enrichment tool for low-abundant protein targets. However, several aspects of the classical IP procedure such as nonspecific protein binding to the isolation matrix, d
Publikováno v:
Methods in Molecular Biology ISBN: 9781493925490
Immunoprecipitation (IP) is commonly used upstream of mass spectrometry (MS) as an enrichment tool for low-abundant protein targets. However, several aspects of the classical IP procedure such as nonspecific protein binding to the isolation matrix, d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7079ae8a087737a51b7bf4fcd2048afd
https://doi.org/10.1007/978-1-4939-2550-6_12
https://doi.org/10.1007/978-1-4939-2550-6_12
Publikováno v:
SLAS Discovery. 9:334-342
Transcription factors are DNA-binding proteins that regulate the expression of specific genes by controlling transcription ini- tiation. Two families of transcription factors, NFκB and AP-1, play pivotal roles in controlling important cellular proce
Publikováno v:
Journal of Biochemical and Biophysical Methods. 57:143-157
Immunoprecipitation (IP) and coimmunoprecipitation (co-IP) are key techniques for studying protein-protein interactions. These methods utilize immobilized Protein A or Protein G to isolate antibody-bound target antigens. The main disadvantage of trad