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Metabolic engineering of Corynebacterium glutamicum for improved l-arginine synthesis by enhancing NADPH supply

Autor: Ruizhi Han, Jin-Jun Dong, Baojun Kan, Milin Zhan, Guochao Xu, Ye Ni

Publikováno v: Journal of Industrial Microbiology and Biotechnology. 46:45-54

Corynebacterium glutamicum SNK 118 was metabolically engineered with improved l-arginine titer. Considering the crucial role of NADPH level in l-arginine production, pntAB (membrane-bound transhydrogenase) and ppnK (NAD+ kinase) were co-expressed to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cd11ca63821a9f114a6901c78cc3ab0
https://doi.org/10.1007/s10295-018-2103-8

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CRISPR-Cpf1-Assisted Engineering of Corynebacterium glutamicum SNK118 for Enhanced L-Ornithine Production by NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase and NADH-Dependent Glutamate Dehydrogenase

Autor: Milin Zhan, Shuxian Wang, Baojun Kan, Hui Liu, Jin-Jun Dong, Ye Ni, Guochao Xu, Ruizhi Han

Publikováno v: Applied biochemistry and biotechnology. 191(3)

Here, Corynebacterium glutamicum SNK118 was metabolically engineered for l-ornithine production through CRISPR-Cpf1-based genome manipulation and plasmid-based heterologous overexpression. Genes argF, argR, and ncgl2228 were deleted to block the degr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d1d003359248ba0fda7f7a95e7fea0d
https://pubmed.ncbi.nlm.nih.gov/31950445

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