Insights into the Mechanism of Installation of 5-Carboxymethylaminomethyl Uridine Hypermodification by tRNA-Modifying Enzymes MnmE and MnmG.

Autor: Bommisetti P; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States., Bandarian V; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.

Publikováno v: Journal of the American Chemical Society [J Am Chem Soc] 2023 Dec 13; Vol. 145 (49), pp. 26947-26961. Date of Electronic Publication: 2023 Dec 05.

Intermolecular electron transfer in radical SAM enzymes as a new paradigm for reductive activation.

Autor: Eastman KAS; University of Utah, Department of Chemistry, Salt Lake City, Utah, USA., Jochimsen AS; University of Utah, Department of Chemistry, Salt Lake City, Utah, USA., Bandarian V; University of Utah, Department of Chemistry, Salt Lake City, Utah, USA. Electronic address: vahe@chem.utah.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2023 Sep; Vol. 299 (9), pp. 105058. Date of Electronic Publication: 2023 Jul 17.

Peptide Selenocysteine Substitutions Reveal Direct Substrate-Enzyme Interactions at Auxiliary Clusters in Radical S -Adenosyl-l-methionine Maturases.

Autor: Rush KW; Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, Oregon 97239, United States.; Department of Chemistry, Reed College, 3203 SE Woodstock Blvd., Portland, Oregon 97202, United States., Eastman KAS; Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, United States., Kincannon WM; Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, United States., Blackburn NJ; Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, Oregon 97239, United States., Bandarian V; Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, United States.

Publikováno v: Journal of the American Chemical Society [J Am Chem Soc] 2023 May 10; Vol. 145 (18), pp. 10167-10177. Date of Electronic Publication: 2023 Apr 27.

Elucidation of the substrate of tRNA-modifying enzymes MnmEG leads to in vitro reconstitution of an evolutionarily conserved uridine hypermodification.

Autor: Bommisetti P; Department of Chemistry, University of Utah, Salt Lake City, Utah, USA., Young A; Soliome Inc, San Francisco, California, USA., Bandarian V; Department of Chemistry, University of Utah, Salt Lake City, Utah, USA. Electronic address: vahe@chem.utah.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2022 Nov; Vol. 298 (11), pp. 102548. Date of Electronic Publication: 2022 Sep 28.

A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence.

Autor: Fleming BA; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Blango MG; Junior Research Group RNA Biology of Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (Leibniz-HKI), 07745 Jena, Germany., Rousek AA; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Kincannon WM; Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA., Tran A; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Lewis AJ; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Russell CW; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Zhou Q; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Baird LM; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA., Barber AE; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Brannon JR; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Beebout CJ; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Bandarian V; Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA., Hadjifrangiskou M; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Howard MT; Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA., Mulvey MA; Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2022 Jul 22; Vol. 50 (13), pp. 7570-7590.