Post-translational modification-centric base editor screens to assess phosphorylation site functionality in high throughput.

Autor: Kennedy PH; Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA.; Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA, USA.; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA., Alborzian Deh Sheikh A; Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA.; Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA, USA.; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA., Balakar M; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Jones AC; Department of Pharmacology, University of California San Diego, San Diego, CA, USA.; Biomedical Sciences Graduate Program, University of California San Diego, San Diego, CA, USA., Olive ME; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Hegde M; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Matias MI; Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA.; Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA, USA.; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA., Pirete N; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Burt R; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Levy J; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Little T; Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA.; Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA, USA.; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA., Hogan PG; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA.; Program in Immunology, University of California San Diego, San Diego, CA, USA.; Moores Cancer Center, University of California San Diego Health, La Jolla, CA, USA., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Doench JG; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Newton AC; Department of Pharmacology, University of California San Diego, San Diego, CA, USA., Gottschalk RA; Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., de Boer CG; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., Alarcón S; La Jolla Institute for Immunology, La Jolla, CA, USA.; AUGenomics, San Diego, CA, USA., Newby GA; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA., Myers SA; Laboratory for Immunochemical Circuits, La Jolla Institute for Immunology, La Jolla, CA, USA. sam@lji.org.; Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA, USA. sam@lji.org.; Division of Signaling and Gene Expression, La Jolla Institute for Immunology, La Jolla, CA, USA. sam@lji.org.; Department of Pharmacology, University of California San Diego, San Diego, CA, USA. sam@lji.org.; Program in Immunology, University of California San Diego, San Diego, CA, USA. sam@lji.org.; Moores Cancer Center, University of California San Diego Health, La Jolla, CA, USA. sam@lji.org.

Publikováno v: Nature methods [Nat Methods] 2024 Jun; Vol. 21 (6), pp. 1033-1043. Date of Electronic Publication: 2024 Apr 29.