An Optogenetic Tool to Raise Intracellular pH in Single Cells and Drive Localized Membrane Dynamics

Autor: Katharine A. White, Caitlin E. T. Donahue, Michael D. Siroky

Publikováno v: Journal of the American Chemical Society

Intracellular pH (pHi) dynamics are critical for regulating normal cell physiology. For example, transient increases in pHi (7.2-7.6) regulate cell behaviors like cell polarization, actin cytoskeleton remodeling, and cell migration. Most studies on p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::821aae6b316751217a47471d5d158978
http://europepmc.org/articles/PMC8603357

Omega Rhodopsins: A Versatile Class of Microbial Rhodopsins

Autor: Soon Kyeong Kwon, Sung-Hoon Jun, Jihyun F. Kim

Publikováno v: Journal of Microbiology and Biotechnology. 30:633-641

Microbial rhodopsins are a superfamily of photoactive membrane proteins with covalently bound retinal cofactor. Isomerization of the retinal chromophore upon absorption of a photon triggers conformational changes of the protein to function as ion pum

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49d50abdaf56a50ecd78b6b6f9ada252
https://doi.org/10.4014/jmb.1912.12010

Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization

Autor: Carol V. Robinson, Hsin-Yung Yen, Juan F. Bada Juarez, Kin Kuan Hoi, Igor Schapiro, Danny Axford, Anthony Vial, Anthony Watts, Isabel Moraes, Suliman Adam, James M. Birch, J. Vinals, Tristan O. C. Kwan, Pierre-Emmanuel Milhiet, Peter J. Judge

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-020-20596-0⟩
Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-020-20596-0⟩
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)

Many transmembrane receptors have a desensitized state, in which they are unable to respond to external stimuli. The family of microbial rhodopsin proteins includes one such group of receptors, whose inactive or dark-adapted (DA) state is established

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9000a783000beccb3e3bcfe84a11468c
https://hal.archives-ouvertes.fr/hal-03415302

Intrinsic photoisomerization dynamics of protonated Schiff-base retinal

Autor: Hjalte V. Kiefer, Pavel A. Kusochek, Jeppe Langeland, Elisabeth Gruber, Lars H. Andersen, Anastasia V. Bochenkova

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Kiefer, H V, Gruber, E, Langeland, J, Kusochek, P A, Bochenkova, A V & Andersen, L H 2019, ' Intrinsic photoisomerization dynamics of protonated Schiff-base retinal ', Nature Communications, vol. 10, no. 1, 1210, pp. 1-9 . https://doi.org/10.1038/s41467-019-09225-7
Nature Communications

The retinal protonated Schiff-base (RPSB) in its all-trans form is found in bacterial rhodopsins, whereas visual rhodopsin proteins host 11-cis RPSB. In both cases, photoexcitation initiates fast isomerization of the retinal chromophore, leading to p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01a9a293080d1e712fb7d4a376d7d604
http://link.springer.com/article/10.1038/s41467-019-09225-7

Constitutive activity of the dual-chromophore photoreceptor Archaerhodopsin 4

Autor: Xiaoyan Ding, Haolin Cui, Anthony Watts, Qixi Mi, Xin Zhao, Chao Sun, Dongxue Liu, Xiao He, Sijin Chen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::072e75f32d39d1824d912174de970ffe
https://doi.org/10.1016/j.bpj.2019.11.850