Zobrazeno 1 - 10
of 746
pro vyhledávání: '"BL21"'
Autor:
Bing Fu, Meng Chen, Xianfeng Bao, Jiajie Lu, Zhiwen Zhu, Fuyao Guan, Chuyang Yan, Peize Wang, Linglin Fu, Ping Yu
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 503-512 (2024)
Vitamin B2 is an essential water-soluble vitamin. For most prokaryotes, a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and FAD. In this study, the plasmid pNEW-AZ containing six key genes for the ri
Externí odkaz:
https://doaj.org/article/4182952355e74a44a23b629e84cead47
Autor:
Heba Shawky, Ashraf A. Tabll, Reem M. Elshenawy, Naiera M. Helmy, Rehab I. Moustafa, Yasser K. Elesnawy, Marwa M. Abdelghany, Yasmine S. El-Abd
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-19 (2024)
Abstract Background Although E. coli is generally a well-opted platform for the overproduction of recombinant antigens as heterologous proteins, the optimization of expression conditions to maximize the yield of functional proteins remains empirical.
Externí odkaz:
https://doaj.org/article/27e208de221445c593edcbf1faf3dd24
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-14 (2023)
Abstract Background Identification and characterization of novel, faithful and processive DNA polymerases is a driving force in the development of DNA amplification methods. Purification of proteins from natural phages is often time-consuming, cumber
Externí odkaz:
https://doaj.org/article/0dd842af746f41e98d79be2e25f95dc2
Publikováno v:
Journal of Kerman University of Medical Sciences, Vol 30, Iss 4, Pp 233-237 (2023)
Introduction: The Human papillomaviruses (HPV) main capsid protein L1 is naturally capable to self-assemble as virus-like particles (VLPs). There are different recombinant protein expression systems such as bacteria, yeast, insect, plant, and mammali
Externí odkaz:
https://doaj.org/article/ba24774e4dc94ba598d7944fdafae47b
Autor:
Le Li, Bingjie Yu, Yingji Lai, Siyuan Shen, Yawei Yan, Guojun Dong, Xiangyun Gao, Yanrong Cao, Caojie Ge, Liqin Zhu, Huan Liu, Shanhui Tao, Zhiang Yao, Shijun Li, Xiaojie Wang, Qi Hui
Publikováno v:
Frontiers in Pharmacology, Vol 14 (2024)
Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional protein that plays a crucial role in various biological processes. In this study, we aim to accomplish the scaling-up production of mature hbFGF (146aa) b
Externí odkaz:
https://doaj.org/article/1b78d3f5875c44e5a222ad2c8aa0f823
Autor:
Klaudia Arauzo-Aguilera, Luisa Buscajoni, Karin Koch, Gary Thompson, Colin Robinson, Matthias Berkemeyer
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-15 (2023)
Abstract Introduction In the biopharmaceutical industry, Escherichia coli is one of the preferred expression hosts for large-scale production of therapeutic proteins. Although increasing the product yield is important, product quality is a major fact
Externí odkaz:
https://doaj.org/article/031358d69e864ff89306bec9adef1611
Autor:
Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson
Publikováno v:
Biomedicines, Vol 12, Iss 6, p 1226 (2024)
The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, w
Externí odkaz:
https://doaj.org/article/2f2c2d43055c4acc9c6058f06ddb984c
Autor:
Marcella de Divitiis, Diletta Ami, Alex Pessina, Alessandro Palmioli, Barbara Sciandrone, Cristina Airoldi, Maria Elena Regonesi, Luca Brambilla, Marina Lotti, Antonino Natalello, Stefania Brocca, Marco Mangiagalli
Publikováno v:
Biotechnology for Biofuels and Bioproducts, Vol 16, Iss 1, Pp 1-19 (2023)
Abstract Background Escherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as IPTG or pure lactose as inducers of heterologous expression. A possible way to reduce the p
Externí odkaz:
https://doaj.org/article/7d0a9741ecdd445fb78e8239c1f5a2fd
Autor:
Lele Li, Hui Li, Qingwu Tian, Baosheng Ge, Xiaotong Xu, Yuanyuan Chi, Huaizhi Zhao, Yanfei Liu, Nan Jia, Tingting Zhou, Yuanqi Zhu, Yusun Zhou
Publikováno v:
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-9 (2022)
Abstract Background Due to its high expression capability, recombination of Escherichia coli and pET vector has become the bioengineering preferred expression system. Because β-lactamases mediate bacterial antimicrobial resistance, these enzymes hav
Externí odkaz:
https://doaj.org/article/2d68d188bf6e463d913e74078b87bf26
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