Zobrazeno 1 - 10
of 792
pro vyhledávání: '"BIOTECHNOLOGY AND BIOENGINEERING"'
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103187- (2024)
Summary: Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithogra
Externí odkaz:
https://doaj.org/article/c34e49a81b8b452f94d1a29d8e6a8e47
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103339- (2024)
Summary: Yeast surface display is a versatile protein engineering technology, enabling precise control of the applied selection pressure. We present a yeast-surface-display-based protocol for the enrichment of binders specifically recognizing ligand-
Externí odkaz:
https://doaj.org/article/4051b930318a46f2a3e27ce3fcb8665d
Autor:
Patricia Kiesler, Stella S. Lee, Alexis L. Norris, Mayumi F. Miller, Carlo J. Mercado, Adam L. Moyer, Samantha Maragh
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103385- (2024)
Summary: Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step to
Externí odkaz:
https://doaj.org/article/c6050da7b94040f6b4e5b04d3c11a92b
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103440- (2024)
Summary: Monoclonal antibodies (mAbs) targeting bacterial virulence factors may represent promising therapeutics in the fight against severe bacterial infections. Here, we present an approach for developing human-derived antibodies targeting the type
Externí odkaz:
https://doaj.org/article/8afc04c295aa467c99145a933872eb6a
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103447- (2024)
Summary: Alpha-synuclein (α-Syn) is an important molecule in the pathogenesis of Parkinson’s and Alzheimer’s disease-related dementias such as Lewy body dementia, forming multiple pathological species. In vitro disease models, including human ne
Externí odkaz:
https://doaj.org/article/ed88c529e2ad4845bbba6c77071a8165
Autor:
Ana Quiroz-Huanca, Ana Sanchez-Castro, Pablo Soriano-Castillo, Chiara Poletti, Therese Manuela Nloh Tientcheu, Attilio Fabbretti, Anna Maria Giuliodori, Pohl Milon
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103448- (2024)
Summary: Here, we present an in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of split-GFP for translation test (FAST), based on the small fragment GFP11 binding to GFP1-10fast. We detail the expression and purifi
Externí odkaz:
https://doaj.org/article/377b6e1fe4954eb4a617591af978f478
Autor:
Huanhuan Lou, Haiying Xiang, Wanli Zeng, Jiarui Jiang, Jianduo Zhang, Li Xu, Chenglu Zhao, Qian Gao, Zhenghe Li
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103437- (2024)
Summary: Plant virus vectors have emerged as promising tools for CRISPR-Cas reagent delivery. Here, we present a protocol for DNA-free plant genome editing using an engineered RNA virus vector for the transient delivery of CRISPR-Cas components. We d
Externí odkaz:
https://doaj.org/article/68347911f2014e04992d7623b555cdf1
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103426- (2024)
Summary: Cement-free and photosynthetic cyanobacterial cell-based living building materials (LBMs) can be manufactured using microbially induced calcium carbonate precipitation (MICP) technology. Here, we present a protocol for cultivating Leptolyngb
Externí odkaz:
https://doaj.org/article/4d69f673865b4ff39a38378e1d0f7b8d
Autor:
Shanshan Duan, Guokang Ma, Junjie Chen, Xuyang Shi, Zishuo Yuan, Wenwen Zhou, Qiuting Deng, Yang Wang, Jianhua Yin, Yue Yuan, Chuanyu Liu
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103430- (2024)
Summary: Single-cell analysis of human peripheral blood cells provides insights into innate and adaptive immune systems. However, robust protocols are essential to ensuring single-cell sequencing data quality and cell viability. Here, we present a pr
Externí odkaz:
https://doaj.org/article/0b2ff2f0919443a7b02998ebe8d41e2e
Autor:
Mehdi Salar Amoli, Linqi Jin, Sarah Rezapourdamanab, Maher Saadeh, Yamini Singh, Liqun Ning, Boeun Hwang, Martin L. Tomov, Christopher N. LaRock, Morteza Mahmoudi, Holly Bauser-Heaton, Vahid Serpooshan
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103393- (2024)
Summary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The r
Externí odkaz:
https://doaj.org/article/f7c78c8e180a497e96ed448a8186e33a